Several genetically engineered mouse models of melanoma using constitutively active and inducible transgenes and germline knockout alleles have studied p16INK4a and melanoma KB Monahan et al 5810 the interaction of Ras activation and p16INK4a/Arf/p53 loss. Mice engineered with a constitutive, ...
a–c, SV40 large-T-antigen-immortalizedATTACMEFs robustly express endogenous p16Ink4a(owing to SV40 large-T-antigen-mediated inactivation of Rb) but fail to engage theATTACtransgene and are not subject to FKBP–Casp8-mediated elimination.a, p16Ink4aprotein levels in passage 4 (P4) primary M...
p16-3MR mice carry a transgene consisting of a trimodal reporter under the regulation of a p16 promoter [18]. p16 is a tumor suppressor gene involved in regulating cellular senescence, specifically through its induction of G1 cell cycle arrest by inhibiting cyclin-dependent kinases [3,19]. ...