Vector containing an enhanced p15a origin of replicationA molecular expression and cloning system which employs an oligonucleotide which converts a low copy number plasmid into a more efficient high copy number plasmid is disclosed. A high copy number plasmid can be used to facilitate more efficient...
Vector pSAM is a pET-28a(+)-derived plasmid with the p15A origin of replication (ori); pET-28a(+) contains the pBR322 replicon that is incompatible with other pBR322-derived plasmids. By replacing the original pET-28a(+) replicon--comprising the ori, RNAI, RNAII, and Rom--with the ...
A high copy number plasmid can be used to facilitate more efficient expression or co-expression of proteins.Sanders, Mitchell C.Hamilton, Maureen
Vector pSAM is a pET-28a(+)-derived plasmid with the p15A origin of replication (ori); pET-28a(+) contains the pBR322 replicon that is incompatible with other pBR322-derived plasmids. By replacing the original pET-28a(+) replicon–comprising the ori, RNAI, RNAII, and Rom–with the ...
Sathiamoorthy S, Shin JA. Boundaries of the Origin of Replication: Creation of A pET-28a-derived Vector with p15A Copy Control Allowing Compatible Coexistence with pET Vectors. PloS One. 2012; 7: 1-7. http://dx.doi.org/10.1371/journal.pone.0047259...