Afterwards, cells were incubated with Alexa633-phalloidin to visualize F-actin (1:300; #A22284; Life Technologies) or DAPI to stain DNA, washed and subsequently mounted using ImmunoMount (#9990402; Thermo Scientific). Imaging was performed on a LSM700 confocal microscope (Zeiss). Image ...
Finally, DRAQ5 was added for DNA stain before analysis by flow cytometry. Histology Tumors were removed from euthanized mice and fixed in 10% formalin overnight, dehydrated and embedded in Tissue Prep 2 paraffin. Samples were sectioned on a rotary microtome, and H&E staining was performed in...
目的探讨宫颈鳞癌患者P120连环蛋白(P120ctn)、β连环蛋白(β-cat)与E钙黏蛋白(E-cad)的表达及生物学意义。 更多例句>> 4) P120ctn 连环蛋白P120 1. To evaluate the expression of P120ctn in non-Hodgkin s lymphoma (NHL) and to explore its clinical significance, immunohistochemistry stain method was ...
To evaluate the expression ofP120ctnin non-Hodgkin s lymphoma (NHL) and to explore its clinical significance, immunohistochemistry stain method was applied to comparatively investigate the protein expression ofP120ctnin paraffin-embedded lymph node tissue slices from 40 cases of NHL and 10 cases of ...
Briefly, the protein SDS-polyacrylamide gel was silver-stained using the SilverSNAP stain for mass spectrometry kit (Pierce). Subsequently, the silver-stained gel bands were excised, destained, reduced, and alkylated with dithiothreitol and iodoacetamide. Proteins were digested for 4 h with 0.6 μ...
At subconfluent growth, immunofluorescent dectection of p120ctn in endothelial cells (EC) shows distribution primarily in the nucleus as confirmed with DAPI stain colocalization. In contrast, in fully confluent monolayers, p120ctn localizes at the plasma membrane with barely detectable expression in ...
Briefly, the protein SDS-polyacrylamide gel was silver-stained using the SilverSNAP stain for mass spectrometry kit (Pierce). Subsequently, the silver-stained gel bands were excised, destained, reduced, and alkylated with dithiothreitol and iodoacetamide. Proteins were digested for 4 h with 0.6 μ...