NEB #名称组分货号储存温度数量浓度 M0660S -20 Nuclease P1 M0660SVIAL -20 1 x 0.1 ml 100,000 units/ml Nuclease P1 Reaction Buffer B0660SVIAL -20 2 x 1 ml 10 X 特性和用法 单位定义 1 单位指在 1X 核酸酶 P1 反应缓冲液中,37℃ 条件下,每分钟消化圆酵母(TorulaYeast)总 RNA 释放 1...
Together, they form a telomeric loop structure with a 3’ TTAGGG overhang to limit nuclease access and to regulate telomerase activity23. The three characterized T. brucei homologs of the mammalian shelterin complex, TbTRF, TbTIF2 and TbRAP1, are involved in VSG expression site regulation. The...
14,15,16,17,18This may enhance the accuracy and efficiency of pre-mRNA processing by facilitating stepwise recruitment of spliceosomal components, such asU1 small nuclear ribonucleoprotein(snRNP) and the U2 auxiliary factor (U2AF) and serine/arginine...
The 5′ site was additionally equipped with restriction sites for XhoI, BglII, and BspEI restriction nuclease and 3′ was with EcoRI, SalI, and KpnI. In the downstream of the 5′ restriction sites, three stop codons were added. Synthesized double strand DNA was digested with XhoI and SalI...