p-Stat3 (B-7) is available conjugated to agarose (sc-8059 AC), 500 µg/0.25 ml agarose in 1 ml, for IP; to HRP (sc-8059 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; to either phycoerythrin (sc-8059 PE), fluorescein (sc-8059 FITC), Alexa Fluor® 488 (sc-8059 AF...
回答者:网友 我前段时间做过,p stat-3的Y705为的磷酸化wb,效果还可以,单一条带而且也很浓。具体我提的是总蛋白,裂解液得是强变性更有利于核蛋白的释放。抗体是1:2000,我用的细胞是胶质瘤细胞系以及肝癌细胞系,本底水平的p-stat3也能测出来。我来回答 提交回答 重置等你来答 水性醛酮树脂CT-3800在水性涂料...
WB (RGB)、IF、IHC(P)、FCM、iBright™ FL1000、FluorChem™、Typhoon、Azureと他の同等システムでRGB蛍光イメージングシステム用のAlexa Fluor® 488、Alexa Fluor® 546、Alexa Fluor® 594 または Alexa Fluor® 647、に共役での利用可能です。 WB (NIR)、IF、FCMとLI-COR®/Odyssey®...
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing. H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, ...
只测stat3和p-stat3在肿瘤细胞中表达有意义吗 我前段时间做过,pstat-3的Y705为的磷酸化wb,效果还可以,单一条带而且也很浓。具体我提的是总蛋白,裂解液得是强变性更有利于核蛋白的释放。抗体是1:2000,我用的细胞是胶质瘤细胞系以及肝癌细胞系,本底水平的p-stat3也能测出
抗体:兔多抗、鼠单抗种类丰富,一抗1万余种,二抗133多种;ELISA、WB、IHC、IF、IP、FC等实验验证应用,进行了 广泛的交叉实验;具有良好的亲合力和特异性; 专业定制 :ELISA试剂盒、抗体、重组蛋白根据您需求可提供专业定制服务。 公司简介 天津睿创生物科技有限公司经过数年的努力发展已迅速成为集产品研发、经营为一...
图中,(A)WB检测HBV‑Tg Stattic,HBV‑Tg Vehicle,及WT Vehicle组肝组织中总Stat3和p‑Stat3蛋白的表达。(B)qPCR检测趋化因子基因(Ccl6,Cxcl1,Cxcl14)和炎症反应基因(Il1b,S100a8,S100a9,S100a11,Saa1,Fgl1)的表达。(A,B)给出了单个小鼠的数值,mRNA的相对表达量和蛋白的相对表达量显示为与WT ...
Applications: Positive Control; Immunogen; SDS-PAGE; WB.(May be suitable for use in other assays to be determined by the end user.)Predicted isoelectric point: 5.7Predicted Molecular Mass: 24.5kDaAccurate Molecular Mass: 25kDa as determined by SDS-PAGE reducing conditions....
PDC-E2 (70 kDa) and pY-STAT3 or STAT3 (both 86 kDa) were detected by Western blot (WB). IP experiments were repeated three times on independent batches of cells. Results PDC‑E2 associates with p‑STAT3 or STAT3 in cholangiocytes and hepatocytes mainly after response to GCDC....
p-Stat3 (Y705) pAb,性能参数:一抗能做WB,IHC,IF等实验,1:500-1:1000左右稀释。说明书在www.bioworlde.com下载!Bioworld再推三大精品现货内参:GAPDH,β-Actin, Tubulin-beta 限时促销!25ul仅380元。现货供应!稀释比达到1:10000.