网络p-羟基苯甲酸酯羟化酶 网络释义 1. p-羟基苯甲酸酯羟化酶 ... 己糖激酶 Hexokinasep-羟基苯甲酸酯羟化酶p-Hydroxybenzoate hydroxylase转化酵素 Invertase ... www.3bio.cn|基于5个网页
The complete primary and tertiary structure of p-hydroxybenzoate hydroxylase is now known. The amino acid sequences of the two largest CNBr peptides have been fitted to the electron-density map at 0.25-nm resolution. The parts of the polypeptide chain contributing the residues to the FAD-binding...
p-Hydroxybenzoate hydroxylase (PHBH) is an FAD-dependent monooxygenase that catalyzes the hydroxylation of p-hydroxybenzoate (pOHB) to 3,4-dihydroxybenzoate in an NADPH-dependent reaction. Two structural features are coupled to control the reactivity of PHBH with NADPH: a proton-transfer network...
By site-directed mutagenesis, Cys-116 was converted to Ser-116 in p-hydroxybenzoate hydroxylase (EC 1.14.13.2) from Pseudomonas fluorescens. In contrast to wild-type enzyme, the C116S mutant is no longer susceptible to oxidation by hydrogen peroxide and shows no reactivity towards 5,5'-dithiobis...
The crystal structure of the reduced form of the enzyme p-hydroxybenzoate hydroxylase from Pseudomonasm fluorescens, complexed with its substrate p-hydroxybenzoate, has been obtained by protein X-ray crystallography. Crystals of the reduced form were prepared by soaking crystals of the oxidized enzyme-...
In order to elucidate the molecular mechanism of the catalytic reaction and enzyme conformation, we substituted 53 conserved residues identified by aligning 92 p-hydroxybenzoate hydroxylase sequences and 19 non-conserved residues selected from crystallographic studies of Pseudomonas fluorescens NBRC14160 p-hy...
Absorption spectra of radical forms of 2,4-dihydroxybenzoic acid,a substrate for p-hydroxybenzoate hydroxylase[J].Journal of Biological Chemistry 1991,266(20).Anderson, R. F., Patel, K. B., and Vojnovic, B. (1991) Absorption Spectra of Radical Forms of 2,4-Dihydrobenzoic Acid, a ...
(2013). Conserved and non-conserved residues and their role in the structure and function of p-hydroxybenzoate hydroxylase. Protein Eng. Des. Sel. 26, ... S Akio - 《Protein Engineering Design & Selection》 被引量: 12发表: 2013年
The side chain of Tyr222 in p-hydroxybenzoate hydroxylase interacts with the carboxy moiety of the substrate. Studies on the Tyr222-->Phe mutant, [F222]p-hydroxybenzoate hydroxylase, have shown that disruption of this interaction hampers the hydroxylation of 4-hydroxybenzoate. Tyr222 is possibly ...
(2009). The effects on lignin structure of overexpression of ferulate 5-hydroxylase in hybrid poplar. Plant Physiol. 150: 621–635, https://doi.org/10.1104/pp.109.137059.Search in Google Scholar Terashima, N., Mori, I., and Kanda, T. (1975). Biosynthesis of p-hydroxybenzoic ...