p-AKT(T308) pAb 现货抗体,性能参数:一抗能做WB,IHC,IF等实验,1:500-1:1000左右稀释。说明书在www.bioworlde.com下载!Bioworld再推三大精品现货内参:GAPDH,β-Actin, Tub
The expression level of Akt and p-Akt-Thr308 in group B was signifcantly lower than in group A (Akt 0.41 ± 0.16 vs 1.12 ± 0.77, P = 0.008; p-Akt-Thr308 0.47 ± 0.19 vs 0.82 ± 0.45, P = 0.036) and the control group (Akt 0.41 ± 0.16 vs 1.03 ± 0.53, P = 0.018; p-...
AKT一般被认为是在质膜上被激活 激活后进胞浆或者入核 首先PDK-1磷酸化AKT的T308位点,此时AKT活性极大增加 但还没有完全活化 需mTOR磷酸化s473位点 s473位点破坏了AKT的疏水性 以上两者使AKT活性充分激活 行使完功能后 T308和S473再被去练酸化 终止AKT信号 检测肯定是两者都检测最好 结果如果只有一个点有差异...
The following antibodies were employed: VCP (#2648), GFP (#2555), p-eIF2α (#9721), eIF2α (#9722), CHOP (#2895), Nrf1(#8052), p-ERK1/2 (#9101), ERK (#9102), p-Akt (S473) (#9271), Akt (#9272), p-Akt (T308) (#9275), p-p70S6K (#9234), p70S6K (#2708), p...
P—PI3K—P110 定位于细胞浆和细胞膜 ,P— AKT—T308定位于细胞浆和细胞核。在高倍镜下取4 个不同的视野,各计数200个细胞,计算 阳性细胞的百 分比:0分为阴性,1分为 阳性细胞数 ≤10%,2分为 11%～50%,3分为51% ～75%,4分为 75%;按染色 强度记分 :0分为无色,1分为浅黄色 ,2分为棕黄色,3...
Here we show that in vitro, TCN-P inhibits neither Akt activity nor the phosphorylation of Akt S473 and T308 by mammalian target of rapamycin or phosphoinositide-dependent kinase 1. However, in intact cells, TCN inhibits EGF-stimulated Akt recruitment to the plasma membrane and phosphorylation ...
Akt phosphorylated wild-type p27 but not p27T157A. In cells transfected with constitutively active AktT308DS473D (PKBDD), p27WT mislocalized to the cytoplasm, but p27T157A was nuclear. In cells with activated Akt, p27WT failed to cause G1 arrest, while the antiproliferative effect of ...
by oncogenes such as ras or erbB2, receptor tyrosine kinases, G proteincoupled receptors, or inactivation of the tumor suppressor gene, PTEN, that normally negativelyregulates this pathway. Akt is a crucial kinase in this pathway that is activated by phosphorylation intheactivationdomain(T308)and...
p27T157A. In cells transfected with constitutively active AktT308DS473D(PKBDD), p27WT mislocalized to the cytoplasm, but p27T157A was nuclear. In cells with activated Akt, p27WT failed to cause G1 arrest, while the antiproliferative effect of p27T157A was not impaired. Cytoplasmic p27...
Here we show that in vitro, TCN-P inhibits neither Akt activity nor the phosphorylation of Akt S473 and T308 by mammalian target of rapamycin or phosphoinositide-dependent kinase 1. However, in intact cells, TCN inhibits EGF-stimulated Akt recruitment to the plasma membrane and phosphorylation ...