Mutation of two lamin A phosphorylation sites, Ser-22 (in QASSTPLS22PTRIT) and Ser-392 (in RLRLSPS392PTSQR), has been reported to prevent nuclear lamina disassembly in mitotic cells [3]. Phosphoepitope-specific antibodies that selectively recognize phosphorylated, but not non-phosphorylated, ...
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To examine whether the Pol λ BRCT domain and its α-helix 1 were required for its interaction with PAXX in cells, we examined interaction of Pol λ-WT, -ΔBRCT and -R57A/L60A (RL) with DNA-bound Ku in vitro and also in cells (Fig. 3d, e, Supplementary Fig. 2e, f). Arg57...
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RL and PC cloned amcv-p19 and performed the agroinfiltrations and ELISA experiments; RL contributed to set up Nef purification protocol; MEV and VC performed Western-blot experiments; GB contributed to set up Nef purification protocol; LN performed statistical analysis; LB performed Mass spectrometry...
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