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high density in 6-well polystyrene culture plates (BD Falcon, NSW, Australia). Following incubation for 24 hr, cells were rinsed 3 times in ice-cold PBS. Ice-cold cell lysis buffer (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1%, v/v, Triton X-100, 1%, w/v, Na deoxy-...
International Journal of Molecular Sciences Article Transgenic Silkworm-Based Silk Gland Bioreactor for Large Scale Production of Bioactive Human Platelet-Derived Growth Factor (PDGF-BB) in Silk Cocoons Wenjing Chen 1,2,3, Feng Wang 1,2,3 ID , Chi Tian 1,2,3, Yuancheng Wang 1,2,3, Sheng ...
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Fragment molecules were initially analyzed as pools of five molecules dissolved at were mixed using a Gilson 21105 mlMiqueidachhanindlDerMwSiOth-Dbu6.ffTerhe(2f0r amgMmeNnta pools contained in phosphate, pH 6.5, 96-well 20 mM plates NaCl, c1o0m%p2oHu2nOd, c5o mncMentDraTtTio...
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IR on an Electrothermal IA spectra (4000–400 cm−1) were recorded in KBr plates on a Perkin-Elmer 2000 FT-IR spectrometer or on a Fourier transform, Bruker VERTEX 70V spectrometer using diamond ATR accessory. Absorption maxima are reported in wavenumbers (cm−1). 1H-NMR and ...
Termites have a ten-segmented abdomen with two plates, the tergites and the sternites.[50] The tenth abdominal segment has a pair of short cerci.[51] There are ten tergites, of which nine are wide and one is elongated.[52] The reproductive organs are similar to those in cockroaches ...
Isolated mouse cardiac fibroblasts were cultured in 96 well plates at 1 × 104 cells/well for 24 h and treated with normal glucose (5 mM), high glucose (25 mM), or high glucose media containing SP at 10, 30, 100, 300, and 1000 nM for 24 h. The assay was performed in duplicate ...
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