每日 ig 给药,治疗 12 周后称重,处死大鼠, 收集血清和棕色脂肪组织,检测血清相关生化指标,测定棕色脂肪组织中解偶联蛋白 1(UCP1)和磷酸化 AKT(p-AKT) 的表达水平.结果 与模型组相比,左卡尼汀 100,200,300 mg/kg 组的总胆固醇(TC),三酰甘油(TG)明显降低,高密 度脂蛋白胆固醇(HDL-C)明显升高(P<0.05);...
mEA-H12741人磷酸二酯酶9A(PDE9A)ELISA试剂盒 mEA-H12742人磷酸化AKT蛋白(p-AKT)ELISA试剂盒 mEA-H12743人磷酸化c-Jun氨基末端激酶(p-JNK)ELISA试剂盒 mEA-H12744人磷酸化HER2蛋白(p-HER2)ELISA试剂盒 mEA-H12745人磷酸化IκB激酶-α(p-IκB-α)ELISA试剂盒 mEA-H12746人磷酸化N-甲基-D-天冬氨酸(p...
【求助】p-Akt压片曝光后为什么是这样的? 最近在做p-Akt的WB,简要步骤如下:用细胞提的蛋白,裂解液RIPA(中),蛋白上样量30ug,丽春红染膜后膜上有蛋白条带,5%BSA室温封闭2h,一抗稀释度1:2000(说明书),用1%BSA的TBST配的,4度过夜,二抗稀释度1:4000,同样是用1%BSA的TBST配的,室温1h,然后曝光。洗膜7min*...
7180 PathScan(R) PDGFR Activity Assay: Phospho-PDGFR, Phospho-SHP2, Phospho-Akt, and Phospho-p44/42 MAPK (Erk1/2) Multiplex Western Detection Kit Antibody Sampler Kit 1 Kit CNY 5,832 9105 Phospho-Bad Antibody Sampler Kit Antibody Sampler Kit 1 Kit CNY 4,200 9369 GSK-3 Antibody Sampler ...
9275L Phospho-Akt (Thr308) Antibody Polyclonal Antibody 300 ul CNY 8,360 9267S Phospho-Akt (Thr450) Antibody Polyclonal Antibody 100 ul CNY 3,487 3343S Phospho-ALK (Tyr1586) Antibody Polyclonal Antibody 100 ul CNY 3,487 3341S Phospho-ALK (Tyr1604) Antibody Polyclonal Antibody 100 ul CNY...
Its effect on calcium flux, cell survival, growth, cell cycle arrest, and apoptosis is mediated through the PI3K and Akt pathways (3-5). Tyr1022 is located in one of the NPXY motifs in SHIP1, and its phosphorylation is important for SHIP1 function (6). Tridandapani, S. et al. (...
Its effect on calcium flux, cell survival, growth, cell cycle arrest, and apoptosis is mediated through the PI3K and Akt pathways (3-5). Tyr1022 is located in one of the NPXY motifs in SHIP1, and its phosphorylation is important for SHIP1 function (6). Background References Tridandapani...
别名AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA 浓度>1mg/ml 免疫原合成P-AKT1(Ser473)多肽 抗体来源兔 特异性抗人 克隆类型多克隆抗体 P-AKT1(Ser473) 抗体产品应用WB 成分亲和纯化抗体,20 mM PBS,150 mM NaCl,100 μg/ml BSA,50%甘油,叠氮化钠<0.02% ...
哦,CST的一抗,说明书上给的推荐剂量是1:2000,我的浓度是1:1000,还需要不需要再把浓度弄高点呢? 66+77 (2013-5-16 16:28:40) 我们刚做了P-AKT,跟你们都是CST的一抗,1:2000稀释,4°过夜,做出来了 jkobn (2013-5-16 16:29:21) 相关疾病: 白血病 === 我们刚做了P-AKT,跟你们都是CST...
显。NF-κB能够与ABCB1基因启动子区域结合。结论：阻断PI3K/AKT信号通路可增强人结肠癌HCT-116/L-OHP 耐药细胞的药物敏感性，抑制p-Akt和p-IκB的磷酸化表达水平，逆转P-gp介导的肠癌多药耐药。 ［关键词］ 结肠癌；多药耐药；P-糖蛋白；磷脂酰肌醇-3-激酶；核转录因子κB DOI: 10.3969/j.issn....