目的:通过观察 3 羟基丁酸、3 羟基己酸嵌段共聚 [poly(3 hydroxybutyrate co 3 hydroxyhexanoate) ,p(3HB co 3HH) ]对犬骨髓干细胞生物学行为的影响 ,探索 p(3HB co 3HH)作为组织工程支架材料在哺乳类动物体内应用的可行性。方法 :应用p(3HB co 3HH)在DMEM培养液中的浸出液 (0 1g/ml)培养犬骨髓干...
This strain can produce P(3HB-co-2 mol% 3HHx) using crude palm kernel oil (CPKO) as a sole carbon substrate. However, the improvement of P(3HB-co-3HHx) copolymer production by this strain has not been studied so far. Thus, this study aims to enhance the production of P(3HB-...
Objective To investigate the biocompatibility of p(3HB-co-3HH) and marrow mesenchymal stell cells (MSCs). Methods MSCs were inoculated to p(3HB-co-3HH), and then cultured for 2-4 weeks in vitro and embedded for 2 weeks in vivo. The growth, proliferation, morphology and phenotype properti...
(3HB-co-3HV) product under the name BiopolTMas a biodegradable thermoplastic material produced using the Gram-negative bacillusAlcaligenes eutrophus. However, the P(3HB-co-3HV) biopolymer displays a hydrophobicity, low degradation rate, inflammatory reaction and low cell proliferation as well as ...
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目的 探讨胚胎干细胞是否适合在聚(3-羟基丁酸酯-co-4-羟基丁酸酯)[P(3HB-co-4HB)]上分化生成心肌补片,添加维生素C能否提高诱导率。 方法 从3只平均体重为37.5 g的清洁级雌性昆明小白鼠胎鼠中提取胚胎纤维状(MEF)细胞,复苏并传代培养小鼠胚胎干细胞(ESC)。小鼠胚胎干细胞来自于上海生命科学院。用悬滴法形成...
在PHAmily的探索之旅中,我们发现了一种颇具潜力的高分子共聚物——P(3HB-co-4HB-co-3HV),我们简称为“PHA三聚物”。这种材料由3-羟基丁酸酯(3HB)、4-羟基丁酸酯(4HB)和3-羟基戊酸酯(3HV)三种单体精心组合而成,听起来可能有点复杂,但别担心,它的特性绝对值得深入了解。同时结合3HB、4HB和3HV...
Furthermore, P(3HB-co-8%-3HV) fibers were prepared by one-step-drawing after the formation of small nuclei by isothermal crystallization near the Tg... T Tanaka,Y Aoyagi,Y Doi,... - 《Seni Gakkaishi》 被引量: 0发表: 2004年 Process and apparatus for forming a layer of a semiconducto...
目的 探讨胚胎干细胞是否适合在聚(3-羟基丁酸酯-co-4-羟基丁酸酯)[P(3HB-co-4HB)]上分化生成心肌补片,添加维生素C能否提高诱导率。 方法 从3只平均体重为37.5 g的清洁级雌性昆明小白鼠胎鼠中提取胚胎纤维状(MEF)细胞,复苏并传代培养小鼠胚胎干细胞(ESC)。小鼠胚胎干细胞来自于上海生命科学院。用悬滴法形成...
本文的研究对象是聚(3羟基丁酸酯-co-4羟基丁酸酯)[P(3HB-co-4HB)],其力学性能类似于PE和PP,随4HB含量增大,材料发生从脆性到韧性的转变,且加工窗口较窄,P (3HB-co-4HB)的结晶性能较差,结晶速度慢,易发生二次结晶,成型收缩率一般为1-2.5%.本文通过差热(DSC)和热重(TGA)分析了P(3HB-co-4HB)的热...