Cells were detached by treatment with Accutase (Sigma-Aldrich, St. Louis, MO, USA), fixed with 2% formalin for 15 min at room temperature and centrifuged. The pellet was resuspended in 1 mL PBS and incubated with FITC-labeledSambucus nigra(SNA) orMaackia amurensis(MAL I) lectin (all from...
The filters were incubated with a polyclonal mouse-anti-LL-37 antibody (1:50, Hycult biotech b.v., Uden, the Netherlands) combined with a polyclonal rabbit anti-myeloperoxidae antibody (1:300, Dako GmbH, Jena, Germany) for 1 h at room temperature. After washing steps, AlexaFluor 488 ...
. The tubes were sealed with rubber stoppers and electrical tape, being careful to avoid bubble formation. They were then submerged in a 32-gallon plastic garbage can of unfiltered seawater (O2concentration: 228.12 µmol l−1) in a dark cold room (8 °C) for 48 h. After ...
(South Korea), National Renewable Energy Laboratory (USA), and Kyungpook National University (South Korea) published together an article that has only 1 citation [14], illustrating that institutional co-authorship is not the only determining factor for the relevance of a publication, but also the...
The electronic structure of strained LSCO films was probed using FY XAS performed at beam line 4-ID-C of the Advanced Photon Source at Argonne National Laboratory. Computational All calculations were performed using DFT within the Vienna Ab Initio Simulation Package (VASP) with a plane-wave basis...
With the advent of the 5G era and the rise of the Internet of Things, various sensors have received unprecedented attention, especially wearable and stretchable sensors in the healthcare field. Here, a stretchable, self-healable, self-adhesive, and room-temperature oxygen sensor with excellent rep...
Seawater samples for 16S rRNA gene analysis were filtered immediately after the retrieval of the Niskin bottles. The filtration was carried out in a temperature-controlled room (2 °C) in the dark and did not exceed 1−1.5 h. Three liters of seawater were filtered onto a 0.22 μm...
The hypothesis of a Leishmania-mediated ROS detoxification during blood meal digestion is currently being studied in our laboratory. An increasing body of sand fly sequence data (30, 31, 47, 48) allows us to infer that the sand fly gut immune response will be similar in overall organization ...
Interestingly, the actual cause and mechanism of superconductivity is extremely complicated, and not known precisely. But researchers in a laboratory would likely take this sample, and use it in either a oxidizing or deoxidizing process to create exactly the composition they desired for whatever ...
Oxygen reacts spontaneously with many organic compounds at or below room temperature in a process called autoxidation.[128] Most of the organic compounds that contain oxygen are not made by direct action of O 2. Organic compounds important in industry and commerce that are made by direct oxidatio...