Briefly, 50-200 ml of exponentially growing ExpiCHO cells were transiently transfected using ExpiFectamine CHO Kit (Thermo Scientific) and were cultured for 8 days according to the manufacturer's instructions “standard protocol” (Thermo Scientific) at 37° C. in a humidified incubator. Cells ...
Based on existing evidence into socio-demographic, familial, and clinical factors25, we grouped variables a priori on the anticipated strength of association with the outcome in decreasing levels of priority26,27. All variables were categorised in this way in a protocol before any statistical analys...