分别将0、10、100、1000μg剂量的鸡卵蛋白(OVA, Grade V, Sigma)加40mg氢氧化铝溶于pH7.4的PBS 0.2ml中,于第0、7、14d分别给4组小鼠腹腔内注射。 第15～20d为雾化激发阶段,将小鼠放入雾化吸入箱,通过雾化发生器雾化吸入5%OVA生理盐水液,每天1次,每次20min,共6d,小鼠出现烦躁不安、呼吸急促、腹肌痉挛、两便失...
and sectioned at a thickness of 4 µm. Sections were stained separately with hematoxylin and eosin and periodic acid-Schiff (PAS) stain. Inflammation was graded using a 0–4 grade scoring system: 0, no inflammation; 1, mild inflammation; 2, moderate inflammation; 3, severe inflammation; ...
货号:1056700 包装:100mL European Pharmacopoeia Reagent Molybdovanadic reagent品牌:Reagecon;订货号:1056700类别:欧洲药典溶液 微信号 15221999938 联系我们 上海金畔生物科技有限公司 电话:021-50837765 手机:15221999938 网址: www.jinpanbio.com Email:sales@jinpanbio.com ...
Commercially available poly(L-lactide) (PLLA) (Sigma) with an average Mw of 160 kDa and poly(D,L-lactide-co-glycolide) (PLGA) (Resomer, type 50 : 50, Boehringer Ingelheim) with Mw of 52 kDa were used throughout this study as received. Chitosan (Mw = 60 kDa; acetylation...
在本发明的另一个优选实施方案中,所述装置是通过与生物素-peg4-炔基(sigma,764213)进行紫外线光聚合而制成的。随后与亲和素:链霉亲和素反应。尽管如此,在将生物体(biotic)连接到柱之前将亲和素连接到生物上是在本发明的范围内的,反之亦然。[0018]在另一个优选实施方案中,所述装置通过使所述抗体与所述链霉...
冷冻保存使用之DMSO 等级, 必须为Tissue culture grade之DMSO (如Sigma D2650), 其本身即为无菌状况, 次开瓶后应立即少量分装于无菌试管中, 保存于4°C, 避免反复冷冻解冻造成DMSO 之裂解而释出有害物质, 并可减少污染之机会。若要过滤DMSO, 则须使用耐DMSO 之Nylon 材质滤膜。15、冷冻保存细胞之方法?冷冻...
in a 0.5 mL suspension of 0.5% methylcellulose (Sigma Chemical Co. St. Louis, MO, USA) and 0.025% Tween 20 (Sigma Chemical Co.) at a dose of 3 mg/kg (SC-560), 100 mg/kg (celecoxib) twice a day, 20 mg/kg (taxol) once a week. The doses of COX inhibitors were chosen for...