Using bioinformatics and genome mining, we identified four loci (lic11623,lic12252,lic12254andlic12258) inL. interrogansencoding hypothetical proteins containing a conserved bacterial surface antigen (D15) domain (IPR000184), a characteristic feature of Omp85 family members (Fig.1A)53. Of the four,...
L. interroganscontains four Omp85 proteins. (A) Interpro (EMBL-EBI) describes four proteins inL. interrogansserovar Copenhageni strain L1-130 proteome containing conserved bacterial surface antigen D15 domains (IPR000184), a characteristic feature of proteins belonging to the Omp85 family. LIC11623,...
Therefore, we deletedrlpAin the temperature-sensitiveE.colistrain LP11-1 (ftsK44) to generate LP11-1 ∆rlpA(Supplementary Fig.S2) and assessed the impact of this deletion on cell growth and morphology. We also created anrlpAknockout in theE.colistrain W3110 to compare the impact ofrlpAde...