OT-II和OT-I小鼠都是T细胞受体（TCR）转基因鼠。OT-II小鼠的CD4 T细胞可以识别OVA抗原（OVA-323-339），而OT-I小鼠的CD8 T细胞可以识别OVA抗原（OVA-257-264）。这些小鼠常被用作研究免疫反应和疫苗的模型。OT-II:(CD4+---ovalbumin 323-339 )These transgenic mice express the mouse alpha-chai...
为了检测抗原特异性CD8 + T细胞,将1x103个初始卵白(OVA)特异性OT-I CD8 + T细胞转移至野生型小鼠,并随后将其移植到表达OVA的Ptgs1/Ptgs2-/-BRAF V600E肿瘤中(Ptgs1/Ptgs2-/-BRAF V600E -OVA)。OT-I细胞产物在第6天很容易在肿瘤中检测到,并且OT-I细胞与cDC1的聚集显著高于多克隆内源性CD8 + T细胞...
这些小鼠携带了小鼠Tcra-V2和Tcrb-V5基因的转基因插入。这些转基因T细胞受体被设计用来识别在H2Kb(与CD8共受体通过MHC类I分子进行相互作用)背景下的卵清蛋白肽段257-264(OVA257-264)。这导致MHC类I限制性的、卵清蛋白特异性的、CD8+ T细胞(OT-I细胞)的产生。也就是说,这种小鼠的CD8 T细胞主要在MHC I分子的...
沉淀用1ml的1x红细胞裂解液重悬细胞,室温静置5min裂解红细胞,随后用10ml预冷的facs缓冲液终止裂解,混匀后,离心得沉淀,用含1nm的n4(siinfekl,ova257-264抗原多肽片段)肽的k medium重悬至2million/ml,铺到6孔板中,每孔1ml即2million/孔。未加多糖组合物的对照组和实验组均添加100iu/ml的il-2(白细胞介素-...
磁珠分选出OT-I和OT-Ⅱ小鼠淋巴结和脾脏中的CD8+T和CD4+T细胞,加入特异性抗原(OVA),再与TEDCs共培养。流式细胞术(FCM)检测细胞表面分子MHC-Ⅱ和共刺激分子... 白煜 - 南京医科大学 被引量: 0发表: 2019年 Arginase I Production in the Tumor Microenvironment by Mature Myeloid Cells Inhibits T-Cell Re...
Creative Biolabs provides Anti-OVA (SIINFEKL) T Cell Receptor (clone OT-I), Jurkat Cell Line product for Biopharmaceutical research,preclinical and clinical trials.
Homing of Tumor-Specific T Cells in the B16OVA/OT-I Model System - towards T Cells as Carriers of Cytotoxic Substances in Therapy of CancermelanomaimmunotherapyuveitisautoimmunityCTLA-4 antibodyCytotoxic T Lymphocyte-associated antigen 4 (CTLA-4) is an important costimultory receptor exp...
Perforin-deficient CTL had a reduced ability to kill OVA-expressing islets in vitro (22.1 ± 3.8%) compared with wild-type CTL (71.4 ± 4.6%). Fas-deficient islets were only slightly protected from wild-type CTL but were completely protected from the residual killing observed with perforin-...
(kako Vodič naziva moja prijateljica Ivana Dimić) i dokaz da GRAĐANI mogu pobijediti ekstremne situacije. U osnovi je individualna, a potom i kolektivna Filozofija adaptiranja na novu normalnost. A to uključuje sabranost, fokus i poštovanje pravila neophodnih za pobjedu ...
Perforin-deficient CTL had a reduced ability to kill OVA-expressing islets in vitro (22.1 +/- 3.8%) compared with wild-type CTL (71.4 +/- 4.6%). Fas-deficient islets were only slightly protected from wild-type CTL but were completely protected from the residual killing observed with ...