infectionMicroRNAs(miRNAs)play important roles in rice response to Magnaporthe oryzae,the causative agent of rice blast disease.Studying the roles of rice miRNAs is of great significance for the disease control.Osa-miR167d belongs to a conserved miRNA family targeting auxin responsive factor(ARF)...
摘要 本发明公开了介导水稻穗轴分化的osa-miR167及其应用。osa-miR167于水稻生殖器官中特异表达,将osa-miR167过表达双元表达载体经农杆菌介导,T-DNA插入并整合到水稻染色体上,所获得的转基因水稻穗轴分化异常,充实率低。该技术可为遗传改造定向创制水稻新种提供一种有效的方法。新闻...
已证实,osa-MIR167a和osa-MIR167h串联形成同一个cluster,而其他基因是单独零星分布于各个染色体。osa-MIR167基因家族各成员茎环结构长度及序列各异,但是经过剪切形成的核心osa-miR167序列只有两种,如SEQ ID NO:3及SEQ ID NO:4所示。osa-MIR167a至osa-MIR167c核心序列为SEQ ID NO:3,osa-MIR167d至osa-MIR167...