A central challenge in expanding the genetic code of cells to incorporate noncanonical amino acids into proteins is the scalable discovery of aminoacyl-tRNA synthetase (aaRS)–tRNA pairs that are orthogonal in their aminoacylation specificity. Here we computationally identify candidate orthogonal tRNAs fro...
Orthogonal aminoacyl tRNA synthetase (aaRS)/tRNA pairs are an efficient tool for the site-specific introduction of para-azido-L-phenylalanine (pAzF), a non-canonical amino acid, into the amber codon of proteins. In the present study, to improve amber suppression by aaRS, random mutagenesis ...
Genetically encoding distinct non-canonical amino acids (ncAAs) into proteins synthesized in cells requires mutually orthogonal aminoacyl-tRNA synthetase (aaRS)/tRNA pairs. The pyrrolysyl-tRNA synthetase/PyltRNA pair from Methanosarcina mazei (Mm) has been engineered to incorporate diverse ncAAs and ...
system. The orthogonal system is composed of un- natural base pair, shift code, unnatural amino acid, orthogonal aminoacyl tRNA synthetase, orthogonal RNA synthetase, promoter and the orthogonal ribosome. These components work corporately or separately in the organism, programming cells with new ...
作者: GP Rédei 摘要: The suppressor tRNA is not a substrate for endogenous aminoacyl-tRNA synthetases, and the mutant aminoacyl-tRNA synthetase aminoacylates only the suppressor tRNA with an unnatural amino acid but no ot DOI: 10.1007/978-1-4020-6754-9_11967 年份: 2008 收藏...
Orthogonal mRNAsOrthogonal mRNAsThe suppressor tRNA is not a substrate for endogenous aminoacyl-tRNA synthetases, and the mutant aminoacyl-tRNA synthetase aminoacylates only the suppressor tRNA with an unnatural amino acid but no otdoi:10.1007/978-1-4020-6754-9_11967George P. RédeiSpringer...
Current methods rely on very few available tRNA/aminoacyl-tRNA synthetase pairs orthogonal in mammalian cells, the pyrrolysyl tRNA/aminoacyl-tRNA synthetase pair from Methanosarcina mazei (Mma PylRS/PylT) being the most active and versatile to date. We found a previously uncharacterized pyrrolysyl...
A single gene of chloroplast origin codes for mitochondrial and chloroplastic methionyl–tRNA synthetase in Arabidopsis thaliana One-fifth of the tRNAs used in plant mitochondrial translation is coded for by chloroplast-derived tRNA genes. To understand how aminoacyl-tRNA synthetases......
The work described in this thesis harnesses the power of residue-specific incorporation to endow proteins with reactive chemical handles that can be modified by azide-alkyne cycloaddition (click chemistry).;Since aminoacyl-tRNA synthetases (aaRS) determine which amino acid gets incorporated into a ...
coli). However, the general and efficient incorporation of multiple distinct ncAAs by O-ribosomes requires scalable strategies for both creating efficiently and specifically translated O-mRNAs, and the compact expression of multiple O-aminoacyl-tRNA synthetase (O-aaRS)/O-tRNA pairs. We automate the...