About 60 years ago, neurons in the mammalian nervous system sensitive to the direction of visual motion were described in the primary visual cortex of the cat1,2and in the retina of the rabbit3,4. Thereafter, investigation of the circuit origins and the neural mechanisms for direction selectivi...
mM Tris pH 9.5, 100 mM NaCl, 50 mM MgCl2, 0.1% Tween 20 and 2% polyvinyl alcohol) until signals were visible. The reaction was stopped in PBST, post-fixed in 10% formalin/PBST, rewashed with PBST, mounted with 40% glycerol, and observed under a light microscope (IX71, Olympus)...
Images were captured with the inverted fluorescent microscope (Olympus CKX53). Spontaneous differentiation of iPSCs into endoderm, meso- derm, and ectoderm Clonal iPSCs were dissociated using 0.05% Trypsin-EDTA and seeded to 96-well low attachment plates (1·103 cells/well) in mES medium without...
Images were acquired using a 100x /NA = 1.4 immersion oil objective (Olympus) on a DeltaVision Elite deconvolution microscope (Applied Precision, GE). A z series was acquired sampling every 0.2 microns for a total of 15-25 sections. The z series was then deconvolved, and a maximum projection...
After washing three times in TBS-Tween, the eggs were mounted on pre-washed glass-plates in Moewiol and observed with a fluorescence microscope Olympus with 60× objective. DNA replication was monitored by incorporation of BrdU. Eggs were fertilized in the presence of 1 mM ATA and 0.1 mg/ml...
Known locally as the water mountain, for millennia Japan’s iconic Mt Fuji has provided safe drinking water to millions of people via a vast network of groundwater and freshwater springs. Groundwater, which is recharged at high elevations, flows down Fuj
Additionally, the collection and voucher specimens were photographed so we could document the diversity of aposematic signalling, the variability of aposematic patterns, and intraspecific variability. These illustrations were taken using a Canon EOS 700D digital camera attached to an Olympus SZX-16 binoc...
10 min, following which the cytoskeleton was stained using TRITC phalloidin (40734ES75, Yeasen Biotechnology, Shanghai, China), nuclei were stained using DAPI (ab104139, Abcam, Cambridge, MA, USA), and exosome uptake into HUVECs was visualized via confocal microscopy (FV10i, Olympus, Tokyo,...