Bioinformatic analyses and aDNA authentication were conducted using sequences generated via shotgun sequencing of Whole Genome Sequencing (WGS) libraries and sequences generated separately via sequencing of enriched libraries. After trimming and merging126the reads, they were aligned127to the Human Referen...
treatment40(https://www.protocols.io/view/non-udg-treated-double-stranded-ancient-dna-librar-3byl47jmzlo5/v1)(https://www.protocols.io/view/full-udg-treated-double-stranded-ancient-dna-libra-5qpvoyq2zg4o/v1)(dataS1). Genetic libraries were indexed and amplified before shotgun sequencing. ...
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venter’s approach is to take all the dna from all the microbes he found in the sargasso sea at any given location and smash it into bits. he then tries to assemble the pieces into complete genomes, applying the same whole-genome shotgun assembly method he relied on when he conquered ...
Genomic DNAs were isolated from the leaves of semi-wild wheat Zang1817 (Triticum aestivumssp.tibetanumShao) using the modified CTAB40and subjected to libraries construction. Two PCR-free paired-end shotgun libraries were constructed using DNA template size of ~470 bp, and sequenced on the Hi...
Both the initial shotgun and target-enriched libraries were single-end sequenced on an Illumina Hiseq 4000 platform (1 × 75 + 8 + 8 cycles). Sequenced reads were demultiplexed allowing one mismatch in each index and further processed using EAGER (v 1.92.54)29. First, adapter...