We demonstrated that O. tsutsugamushi replicated within human monocyte-derived macrophages. Bacteria stimulated the expression of a large number of genes, including type I interferon genes, interferon-stimul
tsutsugamushi is a member of Gram-negative bacteria [3]. The bacteria is approximately 0.5 μm wide and 1.2–3.0 μm long (figure 1) [4]. In electron microscopic observations of antibody-treated O. tsutsugamushi, Silverman suggested the presence of a capsule-like layer on the surface of O...
Obligate intracellular bacteria include the Rickettsiales, an order which includes the arthropod and nematode symbiontWolbachiaas well as a number of human and veterinary pathogens.Orientia tsutsugamushi(Ot, Class Alphaproteobacteria, Order Rickettsiales, Family Rickettsiaceae) causes the mite-borne human ...
O. tsutsugamushicells that induce host cytoskeleton rearrangement are internalized by clathrin-dependent endocytosis, similar to several other intracellular pathogens such asChlamydiaspp. andNeisseria gonorrhoeae(Chu et al.2006). Once internalized, bacteria are contained within clathrin-coated vesicles, which ...
Scrub typhus is a neglected tropical disease that affects one-third of the world’s population. The disease is caused by Orientia tsutsugamushi (OT),
tsutsugamushi and routine IFA require a BSL3 labora- tory and experienced staff, 2) it is also difficult to estab- lish an IFA cutoff titer using the complicated LCA algorithm which requires specially trained experts. The recombinant protein of the most immunodomi- nant and abundant 56 kDa ...
tsutsugamushi has been reported, it was produced as a fusion peptide which creates a number of inherent disadvantages, including reduced immunogenicity due to improper folding of the bacteria polypeptide. To overcome these problems a non-fusion, recombinant poly-peptide from 56 kDa protein was ...
Little is known whether exosomal miRNAs are regulated during scrub typhus, a potentially lethal infection caused by intracellular bacteria, Orientiatsutsugamushi. In the present study, we utilized a scrub typhus mouse model and collected serum at various time points post infection. A custom ...
Little is known whether exosomal miRNAs are regulated during scrub typhus, a potentially lethal infection caused by intracellular bacteria, Orientiatsutsugamushi. In the present study, we utilized a scrub typhus mouse model and collected serum at various time points post infection. A custom ...
A recombinant, refolded non-fusion polypeptide expressed from a truncated r56 gene of the causative agent of scrub typhus, Orientia tsutsugamushi for the Karp, Kato and Gilli