5a). Eventually a semi-automatic procedure was chosen, which consisted in moving the filter plate with cells to a liquid nitrogen bath outside of the TCP, quickly incubating in −20 oC and then subsequently adding cold ACN and incubating again at −20 oC. The setup was chosen also...
For example, some extremophilic bacteria found in hydrothermal vents can use carbon dioxide as a carbon source for chemosynthesis, which is essential for the food chain in these deep-sea environments. Underground Discovery In the 1960s, researchers began studying extremophiles in places like the ...
Indeed, the way biologists think about the whole idea of "species" when they study these micro-organisms is beginning to shift rapidly. 事实上,生物学家在研究微生物时所认为的“种属”的概念也在迅速的改变着。 www.ecocn.org 5. Micro-organisms able to metabolise sulphur and nitrogen have the po...
Global expansion of urban areas in recent years has induced substantial changes in soil physicochemical characteristics which in turn influence soil biota. Urbanization causes land-use transitions through which fragmentation of soil habitats is inevitable [5,8]. This fragmentation, from large continuous n...
Current understanding of the carbon cycle in methanogenic environments involves trophic interactions such as interspecies H2 transfer between organotrophs and methanogens. However, many metabolic processes are thermodynamically sensitive to H2 accumulati
And, along the way, farmers switched from legumes…to animal feeds more suited to intensive, large-scale animal production. And the excess nitrogen, once trapped by these cover crops, either washed away in the next big rain or went down into the groundwater and, either way, eventually ended...
(1.2 ~ 2.8 Pa H2) are very close to the minimum hydrogen threshold that conventional H2-utilizing CO2-reducing methanogenesis can use (1.7 Pa H2), which is often lower than bulk H2concentrations detected in reactors (< 10 Pa) [25,47]. Thus, we expect that the species performing...
It is a real challenge to use them in biohybrid composites and harness all their advantageous properties. This way it is possible to build environmentally friendly biodegradable items like drug delivery systems, biosensors. In addition, it is also probable to use them for special (opto)electronic ...
Shoots and leaves were harvested at three weeks and ground in liquid nitrogen using a mortar and pestle. The complete protocol is described in the 'Preparing Arabidopsis Genomic DNA for Size-Selected ~20 kb SMRTbell™ Libraries' protocol35. This protocol can be used to prepare purified ...
On the medium used, the nitrogen source is depleted before the carbon source (D-glucose), and it is probably N-limitation which regulates development. In an excellent descriptive account of basidiocarp development in Flamrnulina eel utipes illustrated by S.E.M. and T.E.M. photographs, ...