Reversed-phase HPLC - Organic modifiers - Low viscosity solvents - Gradient elutionThree new organic modifier solvent systems for reversed-phase HPLC are recommended which can increase the speed of analysis up to twofold over conventional modifier systems, without a significant sacrifice in general ...
Reevaluation of ethanol as organic modifier for use in HPLC-RP mobile phases. Journal of the Brazilian Chemical Society, v. 15, p. 300-306, 2004.Ribeiro R. L. V,Bottoli C. B. G,Collins K. E.et al.Reevaluation of Ethanol as OrganicModifier for Use in HPLC-RP Mobile Phases. J. ...
Retention Dependence on Organic Modifier and Interaction Reagent Concentration in Reversed-Phase Ion-Interaction HPLCvitamin Ebeta-carotenemouth mucosasmokingThe effect played on ion-interaction chromatographic retention by the concentrations of the organic modifier and of the ion-interaction reagent as a ...
The purpose of this work was to test the applicability of the current theory to predict the peak retention time and the peak width in the combined pH/organic modifier gradient reversed phase high performance liquid chromatography (RP HPLC). A series of 38 isocratic measurements have been conducted...
Phase ratio Φ for an HPLC column is a common parameter being defined as the ratio between the volume of the stationary phase Vst and the void volume of the column V0. Although apparently simple, the evaluation of phase ratio presents difficulties because there is no sharp boundary between the...
The effect of organic modifier (methanol or acetone) concentration and stationary phase (RPNH2 or RP-CN) properties on solute retention was investigated and the dependence of chromatographic peak shape and symmetry on the mobile and stationary phases used was analyzed. The studies show the ...
(HPLC)techniqueswithhighseparationca-pacityandelevatedsensitivityhavealsofoundemploymentintheanalysisofbarbiturates[3].Thus,thesuccessfuluseofion-pairchromatography[4],micellarHPLC[5],3-(1,8-naphtalimido)propyl-modifiedsilica[6]hasbeenre-ported.Theadvantageousretentioncharacteristicsofporousgraphitizedcarbon(...
美国Rheodyne公司的六通阀进样器最为通用,各大HPLC仪器制造商均以此产品作为仪器的进样器。 工作原理: 1、手柄位进样(Load)位置时,样品经微量进样针从进样孔注射进定量环,定量环充满后,多余样品从放空孔排出; 2、将手柄转动至进样(Inject)位置时,阀与液相流路接通,由泵输送的流动相冲洗定量环,推动样品进入...
To elucidate the underlying mechanisms of these observed effects, other neutral (β-CD, hydroxypropyl-β-CD, and trimethyl-β-CD) as well as chargeable (carboxyethyl-β-CD and succinyl-β-CD) CD derivatives were also tested with MeOH as organic modifier. It can be concluded that the ...
The degree of ionization of the analytes and the type and concentration of organic modifier used were also seen to influence the analytes1 enantio-selectivity and capacity values. © 1996 Wiley-Liss, Inc. 展开 关键词: cyclodextrin chiral propranolol chromatography stationary phase DOI: 10.1002/...