supernatant was removed and 1 ml of dissociation medium (L15 medium (Thermo Fischer Scientific, cat# 21,083,027), 10% FBS, 10 µg/ml DNAse I (diluted 1:1000, VWR International, cat#A3778.0010)) was added
Spinocerebellar ataxia (SCA) subtypes are often caused by expansions in non-coding regions of genes like SCA8, SCA10, SCA12 and SCA36. Other ataxias are known to be associated with repeat expansions such as fragile X-associated tremor ataxia syndrome (FX
The most common inherited cause of two genetically and clinico-pathologically overlapping neurodegenerative diseases, amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), is the presence of expanded GGGGCC intronic hexanucleotide repeat
Yan Zheng and Yongping Shao conceived and designed the experiment; Caifeng Chen, Na Wu, Qiqi Duan and Huizi Yang performed the experiment; Peiwen Yang and Mengdi Zhang helped analysed the results; Caifeng Chen wrote the main manuscript text; Jiankang Liu and Zhi Liu revised the manuscript. Al...
Supplementary Text and Figures Supplementary Figures 1–5 and Supplementary Methods (PDF 9351 kb) Supplementary Movie 1 Flexibility and domain movements in the L1ORF1p trimer. While rotating once around the pseudo–three-fold axis of the trimer, the movie smoothly interpolates between the structural...
It was reported that the autophagosome-associated Rab2A (refer to Rab2 throughout the text) and lysosome-associated Rab7A (refer to Rab7 throughout the text) are involved in the autophagosome-lysosome fusion process22,24,25. We aimed to demonstrate biochemically that these two Rabs could ...
Close Plain text Look Inside Share Share this content on Facebook Share this content on Twitter Share this content on LinkedIn Within this Article Introduction Materials and methods Results Discussion References References Other actions Export citations Register for Journal Updates About...
The (G4C2)n nucleotide repeat expansion (NRE) mutation in C9orf72 is the most common genetic cause of ALS and FTD. The biological functions of C9orf72 are becoming understood, but it is unclear if this gene is regulated in a neural-specific manner. Neuronal activity is a crucial modifier...
We observed that only 135 genes were differentially expressed in wtKO cells compared with WT (Figure 3C). Although there was some overlap with the genes altered in C9 versus C9GC (Figure S6B), gene ontology analysis could not identify any significantly enriched terms. This suggests that C9ORF...
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