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Serum is the most important body fluid and is full of small peptides. In this study, we have established a small ORF-encoded peptides (SEPs) database from mouse GENCODE release. This database provides about half a million putative translated SEPs in mouse. We also extract serum proteins ...
et al. Full ablation of C9orf72 in mice causes immune system-related pathology and neoplastic events but no motor neuron defects. Acta Neuropathol. 132, 145–147 (2016). Article PubMed CAS Google Scholar Burberry, A. et al. Loss-of-function mutations in the C9ORF72 mouse ortholog cause...
targeted full-length sequencing methods, in which pools of biotinylated oligos are used to enrich for full-length cDNAs for sequencing, have characterized focused subsets of the transcriptome with great depth and accuracy27,28. Here, we establish that ORF Capture...
Clavulanic acid, a beta-lactamase inhibitor: biosynthesis and molecular genetics Appl Microbiol Biotechnol, 54 (2000), pp. 467-475 View in ScopusGoogle Scholar Lonetto et al., 1994 M.A. Lonetto, K.L. Brown, K.E. Rudd, M.J. Buttner Analysis of the Streptomyces coelicolor sigE gene rev...
Full size image Next, to investigate MN degeneration/survival, we employed two different approaches. First, we used the CellTiter-Glo® assay to measure metabolically active cells based on the quantification of cellular ATP levels (Fig.3d, top panel). Second, we performed immunostaining for cleav...
Download: Download full-size image Fig. 2. Biochemical parameters in the sera of CMs immunized with recombinant p551 protein (n = 4) and in placebo group (n = 2) after immunization and HEV challenge. Values for alanine aminotransferase (A), aspartate aminotransferase (B), lactate dehydrogenase...
Genetics. 2018;210:357–96. Bate M. The embryonic development of larval muscles in Drosophila. Development. 1990;110:791–804. CAS PubMed Google Scholar Landgraf M, Jeffrey V, Fujioka M, Jaynes JB, Bate M. Embryonic origins of a motor system: motor dendrites form a myotopic map in ...
Full size image Poly-GR and poly-PR proximity proteomes overlap and are highly enriched for ribosomal and nucleolar proteins Analysis of the GO terms and KEGG pathway enrichment was performed in each of the obtained sets of DPR interactors, showing distinct functional classifications for the poly-...
We thank the Caenorhabditis Genetics Center (CGC), which is funded by the NIH Office of Research Infrastructure Programs (P40 OD010440), for providing strains. The antibodies in the MSD assay that detect poly-GA in human iPSC-derived motor neurons were used at Biogen, and were discovered by...