A good nucleophile is bigger in size, readily polarizable and has less electronegativity value. A more electronegative atom holds the lone pair electron towards itself and does not readily attack electrophiles. Answer and Explanation:1 As the size of the anion increases, the polarizability increases...
After 5 min of activation, L-alanine ethyl ester hydrochloride (406 mg, 2.64 mmol) was added and the reaction mixture was stirred for 3 h. The solvent was removed under reduced pressure and the residue was redissolved in CH2Cl2 (30 ml) and washed with a saturated Na2CO3 aqueous solution ...
Two deuterium atoms, one involved in a conserved hydrogen bond between Gly120-NH and FAD-N5 and one involved in a hydrogen bond between the conserved water molecule, Wat541, and the proposed base (Glu361) in the substrate oxidation reaction, were clearly observed in the nuclear difference ...
[45,46]. The substratesacetylcholineandacetylthiocholineare cationic compounds, while the active site of AChE has several anionic residues. Therefore, salts can modulate the interaction of them and ultimately the kinetics of the reaction. Besides the considerations of the charges in the enzyme ...
1. (a) How will the value of Ecell change in an electrochemical cell involving the following reaction of the concentration of Ag+ (aq) is increased ? (b) What will be e. m. f. when the cell reaches equilibrium : Ans. (a) Mg (s) + 2 Ag+ (aq) ——→ Mg2+ (aq) + Ag (...
Before use, the protein was solubilized in 10 mM phosphate-HCl buffer, pH 7, containing 0 or 0.1 M NaCl. The buffer was prepared in 2H2O for infrared experiments. Protein concentration was determined by absorption spectroscopy (ε280 1.0 mL mg− 1 cm− 1 [27]). 2.3. Preparation of ...
The reaction was also carried out under high frequency sonication for 2 hr. THF was removed under reduced pressure of 150 mm Hg using a vacuum pump, washed twice with THF and the solvent was removed using a vacuum pump. The product was air dried and passed through ;The matrix tablets ...
Briefly, total glycans extracted from ascidians tissues (~20 μg) were incubated with 5 μL of fresh generated HNO2 at room temperature for 90 min. The reaction mixtures were then neutralized with 1.0 M Na2CO3. Intact and nitrous acid-degraded glycans were analyzed by agarose gel ...
The conversion of acetyl-CoA to ethanol is carried out in a two-step reaction performed by the two catalytic domains of AdhE. The N-terminal aldehyde dehydrogenase (ALDH) domain converts acetyl-CoA to acetaldehyde, which is then converted to ethanol by the C-terminal alcohol dehydrogenase (...
Cystinuria is a genetic disorder characterized by overexcretion of dibasic amino acids and cystine, causing recurrent kidney stones and kidney failure. Mutations of the regulatory glycoprotein rBAT and the amino acid transporter b0,+AT, which constitute