Flash frozen cortices (10-15 mg) were homogenized in 500-750 μl polysome lysis buffer (100 mM KCl, 5 mM MgCl2, 10 mM Tris-HCl, pH9, 1% Triton X-100, 1% sodium deoxycholate). Extracts were clarified by centrifugation at 2,500 g for 15 minutes at 4°C and cycloheximide and ...