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17 PCR products were gel purified (Qiagen, Hilden, Germany), and library preparation was performed according to the manufacturer's instructions (NEB Next Ultra II DNA Library Prep Kit for Illumina; NEB, Ipswich, MA). Paired-end NGS was performed with the MiSeq Reagent Kit v3 (2 × 300 ...
Complete mitochondrial DNA sequence and phylogenetic analysis of Zhikong scallop Chlamys farreri (Bivalvia: Pectinidae). Mol. Biol. Rep. 38, 3067–3074 (2011). 22. Wu, X. Y., Xu, X. D., Yu, Z. N., Wei, Z. P. & Xia, J. J. Comparison of seven Crassostrea mitogenomes and ...
Total RNA was isolated using the RNAqueous-4PCR Kit (Ambion) according to the manufacturer's instructions. Due to the inclusion of DNase I in the RNAqueous-4PCR Kit (Ambion), the total RNA isolated with this kit is DNA-free. The concentration of RNA samples was ascertained by measuring ...
We analyse sequence data in comparison with mitochondrial genomes of various Bilateria to evaluate the phylogenetic position of Sipuncula. In addition we compare gene order among Lophotrochozoa and evaluate the most parsimonious explanation for gene order changes....