RNA-binding proteins (RBPs) play an essential role in regulating the function of RNAs in a cellular context, but our ability to control RBP activity in time and space is limited. Here, we describe the engineering of LicV, a photoswitchable RBP that binds to a specific RNA sequence in resp...
RNA-binding proteins (RBPs) play an essential role in regulating the function of RNAs in a cellular context, but our ability to control RBP activity in time and space is limited. Here, we describe the engineering of LicV, a photoswitchable RBP that binds to a specific RNA sequence in resp...
Despite efforts to visualize the spatio–temporal dynamics of single messenger RNAs, the ability to precisely control their function has lagged. This study presents an optogenetic approach for manipulating the localization and translation of specific mRNAs by trapping them in clusters. This clustering gre...
Despite efforts to visualize the spatio–temporal dynamics of single messenger RNAs, the ability to precisely control their function has lagged. This study presents an optogenetic approach for manipulating the localization and translation of specific mRNAs by trapping them in clusters. This clustering ...
To obtain precise temporal control of nuclear protein function in embryos, we set out to establish a generalizable, efficient, and highly dynamic optogenetic system. We explored the potential of LEXY (light-inducible nuclear export system) to function in embryos, a blue-light-inducible system shown...
Taking advantages of the precise temporal control of optoRAF1, we attempted to determine if astrocytogenesis depends on the total duration of RAF1 activation. To do so, we lowered the frequency of RAF1 activation by administrating a pattern oflight stimulationwith extended dark period (20-min-...
The cGAS-STING signaling pathway has emerged as a promising target for immunotherapy development. Here, we introduce a light-sensitive optogenetic device for control of the cGAS/STING signaling to conditionally modulate innate immunity, called ‘light-in
Optogenetic and transcriptomic interrogation of enhanced muscle function in the paralyzed mouse whisker padChR2denervationmotor controlmovement disordersmystacial vibrissaeoptogeneticsparalysisplasticityRNA-seqsensorimotor integrationThe functional state of denervated muscle is a critical factor in the ability to ...
Optogenetic control of RNA function and metabolism using engineered light-switchable RNA-binding proteins Article 03 January 2022 Introduction Bacillus subtilis is a model organism for studying how time-varying (dynamic), heterogeneous, and spatially-coordinated gene expression signals control single- and...
Our findings demonstrate specialized properties of the CENP-E (kinesin-7) motor for directional chromosome transport to the spindle equator and for maintenance of metaphase alignment. This work establishes a foundation for optogenetic control of kinetochore function, which is broadly applicable to ...