OptKnock方法基于基因敲除和选择压力的结合,通过选择性地敲除关键基因,影响微生物的代谢网络,使其能够利用原本无法利用的底物。 以下是OptKnock方法的简要步骤: 1.确定目标代谢途径和关键基因:首先,需要确定希望在微生物中创建或增强的新代谢途径,并确定控制该途径的关键基因。这些基因通常是酶编码基因,控制着代谢途
There seems to be aproblem with optKnock. If I run the example code from the cobra protocol model = readCbModel('ecoli_textbook'); model = changeRxnBounds(model, {'EX_o2(e)', 'EX_glc(e)'}, [0 -20], 'l'); selectedRxns = {model.rxns{ [1, 3...
Differential bees flux balance analysis with optknock for in silico microbial strains optimization. PLoS One 9 (7), e102744.Y. Choon, M. Mohamad, S. Deris, R. Illias, C. Chong, L. Chai, S. Omatu, and J. Corchado, "Differential bees flux balance analysis with optknock for in silico...
求翻译:OptKnock是什么意思?待解决 悬赏分:1 - 离问题结束还有 OptKnock问题补充:匿名 2013-05-23 12:21:38 OptKnock 匿名 2013-05-23 12:23:18 正在翻译,请等待... 匿名 2013-05-23 12:24:58 OptKnock 匿名 2013-05-23 12:26:38 OptKnock 匿名 2013-05-23 12:28:18 OptKnock...
Exploring the overproduction of amino acids using the bilevel optimization framework OptKnock. Biotechnol Bioeng. 2003; 84 :887–899. doi: 10.1002/bit.10857. [ Cross Ref ]Pharkya P, Burgard AP et al (2003) Exploring the overproduction of amino acids using the bilevel optimization framework ...
Overall framework of DBFBA with OptKnock.Yee Wen ChoonMohd Saberi MohamadSafaai DerisRosli Md. IlliasChuii Khim ChongLian En ChaiSigeru OmatuJuan Manuel Corchado
Burgard AP, Pharkya P, Maranas CD (2003) Optknock: A bilevel programming framework for identifying gene knockout strategies for microbial strain optimization. Biotechnology and Bioengineering 84: 647-657.Burgard AP, Pharkya P, Maranas CD (2003) Optknock: a bilevel programming framework for ...
Comparison of the minimum guaranteed fluxes from OptKnock, OptReg and OptForce procedures for succinate production in E. coli.Sridhar, RanganathanPatrick, F. SuthersCostas D., Maranas
A hybrid of bees algorithm and flux balance analysis with OptKnock as a platform for in silico optimization of microbial strainsdoi:10.1007/s00449-013-1019-yYee ChoonMohd MohamadSafaai Bin DerisLian En Chai
Here, we generated a SfABCB1 knockout strain of the S. frugiperda using the CRISPR/Cas9 system to explore its potential roles in determining susceptibility to chemical insecticides or Bt toxins. Bioassay results showed that the susceptibility of SfABCB1 knockout strain to beta-cypermethrin, ...