The present invention relates to a method for analysing microbial growth and capable of correcting optical density readings and calculating kinetic growth parameters of one or more micro-organism cultures. The data analysis obtained makes it possible to plot individual growth curves (or growth curves ...
Similar Δ OD (the change in optical density from the initial value to the value where the final population density is reached) responses are observed for temperature and water activity (adjusted using sodium chloride). Over most of the growth permissive ranges, the Δ OD remains high for both...
and simple method of estimating cell density in the absence of other solids or light-absorbing compounds. The use of optical methods for medium containing hydrolysates of woody biomass may not be ideal as plant cell fragments, and other particles can interfere in the same way as microbial cells...
The speed at which a given transparent medium transmits light waves is related to its optical density (not to be confused with mass or weight density). In general, a ray is refracted toward the normal when it passes into a denser medium and away from the normal when it passes into a ...
is the sum of these properties that is measured. It is therefore debatable what term should be applied.ABSORBANCEis sometimes used interchangeably with optical density, but this is not strictly correct. Optical density is generally accepted as the more appropriate term when measuring microbial growth...
2. Biology: Optical density is used to measure the growth of microorganisms in liquid cultures. By measuring the absorbance at specific wavelengths, the density of the microbial population can be determined. 3. Physics: Optical density is used to measure the transparency of materials, such as gla...
aeruginosa, respectively, based on the maximal optical density (OD490 of about 4) being reliably measured by the optical spectrometer. Statistical evaluation. For each sample the adhesion value was calculated by subtracting the value of tex- tiles treated without bacteria from the arithmetic mean...
Abstract Sustainable and effective means to control flying insect vectors are critically needed, especially with widespread insecticide resistance and global climate change. Understanding and controlling vectors requires accurate information about their movement and activity, which is often lacking. The Photonic...
Cells were plated at a density of 50 k/cm2, calibrated by a Trypan blue stain and accounting for the lot plating efficiency specified by the manufacturer. Cells were plated in just the recessed region of the glass-bottomed dish onto the freshly printed MOSAIC arrays and allowed to attach ...
Hippocampi were pooled together, trypsinized for 15 min, briefly incubated in 20% fetal bovine serum, and then plated in glass-bottom 35-mm dishes (GWST-3522, WillCo Wells) coated with 100 µg/ml poly-l-lysine (P2636; Sigma-Aldrich) at a density of ~325,000 cells per dish...