HMGB1 release is another distinctive marker to differentiate between necrosis and pyroptosis.Y. pestisinfection or YopM transfection triggered significant HMGB1 release (Figure 5candSupplementary Figure S3B), while knocking down of NLRP3 blocked YopM-induced cell death and decreased HMGB1 release (Figure...
HMGB1 release is another distinctive marker to differentiate between necrosis and pyroptosis. Y. pestis infection or YopM transfection triggered significant HMGB1 release (Figure 5c and Supplementary Figure S3B), while knocking down of NLRP3 blocked YopM-induced cell death and decreased HMGB1 release ...
Despite assembly of this NLR-ASC “preinflammasome” scaffold during infection (Figure S3A), caspase-1 is not recruited into foci in the presence of YopM delivered by Ypstb (Figures 4E and 4F). Caspase-1 was recruited to the inflammasome in the presence of YopM271A, the mutant that ...
Yersinia yopM mutant-induced pyrin inflammasome activation was significantly reduced when both YopE and YopT were inactivated (Figures 3A–3C), which corresponded to RhoA activation (Figure S3), while an intermediate phenotype was seen with each individual mutant (Figures 3A–3C). In addition, ...
type:s3 config: bucket:thanos endpoint:minio.example.choerodon.io access_key:AKIAIOSFODNN7EXAMPLE secret_key:wJalrXUtnFEMI/K7MDENG/bPxRfiCYEXAMPLEKEY insecure:true signature_version2:false Copy 创建存储的 secret: kubectl -n monitoring create secret generic thanos-objectstorage --from-file=thanos....