yopM蛋白;高活性;yopM protein 纯度::Greater than 90% as determined by SDS-PAGE. 基因名::yopM Uniprot No.::P17778 别名::yopM; yop48; YPCD1.26c; y5054; y0059; YP_pCD60; Outer membrane protein YopM 种属::Yersinia pestis 蛋白长度::Full Length ...
Bacterial Outer Membrane ProteinsThe YopM virulence protein of Yersinia pestis has been described as binding human 伪-thrombin and inhibiting thrombin-induced platelet aggregation in vitro . However, recent studies have shown that a YopM鈥揅yaA fusion protein could be targeted vectorially into ...
Accordingly, the graphiumins were evaluated in our in-house assays; antimicrobial assay,35 mammalian cell cycle assay,36 lipid metabolism assay37 and bone morphogenetic protein-induced alkaline phosphatase expression assay.38 However, they showed no activity in these assays even at 25 μg ml − ...
Immunoprecipitation was performed on 1 μg of protein with anti-NLRP3 antibody. After transfer, the membrane was denatured in 6 M guanidine-HCl solution (6 M guanidine-HCl, 20 mM Tris-HCl, pH 7.5, 1 mM phenylmethylsulfonyl fluoride, and 5 mM dithiothreitol) for 30 min at room temperature...
Yersinia pestis uses type III effector proteins to target eukaryotic signaling systems. The Yersinia outer protein (Yop) M effector from the Y . pestis strain is a critical virulence determinant; however, its role in Y. pestis pathogenesis is just beginn
Protein Purification E. coli strains harboring pGEX-2T vectors encoding YopM sequences from Y. pseudotuberculosis 32777, Y. pestis KIM, and Pestoides A, or Y. enterocolitica 8081 were expressed and purified, as previously described (Chung et al., 2014). GST Pulldowns To construct a vector fo...
The endoplasmic reticulum (ER) integral membrane protein VAP is a common component of membrane contact sites involved in both tethering and lipid transfer by binding directly to proteins containing an FFAT (two phenylalanines [FF] in an acidic tract [AT]) motif. Sequence analyses revealed that ...
The yersinia outer protein M (YopM) is an effector protein of Yersinia species that is able to enter host cells by membrane penetration. In the cell YopM mediates down-regulation of inflammatory responses. We investigated whether YopM has the potential to act as a "selfdelivering" immune ...
The Yersinia outer protein M (YopM) is an effector protein of pathogenic Yersinia and is able to enter host cells by membrane penetration and subsequent downregulation of inflammatory responses. Here, we investigated whether YopM has the potential to act as a 'self-delivering' immune therapeutic ...
This transformation is most likely due to the combination of cytokines, growth factors and cellular interaction, which leads to the stable activated phenotype.YopM is the outer protein M of Yersinia species. It has cell penetrating properties and can translocate into the cell by its two N-...