shortens by about two helical turns in the Open-state structure. While the resolution at the intracellular end of the S4 helix in the Intermediate state precludes definitive assignment of secondary structure, the weaker density at the C-terminal end suggests that it may be on the pathway to ...
Methods Homology Modeling. Homology models of the full-length mouse Kir2.1 channel were performed using the SWISS-MODEL server28–30. The target sequences were taken from Genbank (http://www.ncbi. nlm.nih.gov/Genbank/). The template structures were the chicken Kir2.2 (PDB code: 3JYC...
The gating of Kir channels depends critically on phosphatidylinositol 4,5-bisphosphate (PIP2), but the detailed mechanism by which PIP2 regulates Kir channels remains obscure. Here, we performed a series of Targeted molecular dynamics simulations on the