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www.nature.com/scientificreports OPEN The human amniotic fluid stem cell secretome effectively counteracts doxorubicin-induced cardiotoxicity received: 15 February 2016 accepted: 27 June 2016 Published: 21 July 2016 Edoardo Lazzarini1,*, Carolina Balbi2,*, Paola Altieri1, Ulrich Pfeffer3, ...
The transcript levels of UCP1, DIO2 and PRDM16 were quan- tified in duplicate by quantitative real time polymerase chain reaction (qPCR) on a StepOne Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) using SYBR® Select Master Mix (Applied Biosystems) and specific primers ...
These spectroscopic changes are similar to the ones that we have previously reported between for the aAROemcoomieptyleaxnrdeltahteedpywriatzhoRlyel--Cdi3a,mRien-Ce c5haenldatRore-aCn8d. This complex presented a acted as a "perfect" DNA binutteyrlceanliactolirn17k.eTra(kCe4n) together, ...
coli system ( K0 = 102kBT for the real cells; κ0 = 12kBT for GUVs made of E. coli lipids). The circular areas represent SDs from the single-cell data (accounting for cell variability; variances in the GUVs' data are considerably smaller). The straight line plotted in grey ...
In the case of the native tis- sue sample (temporomandibular joint disc (TMJ disc)) presented in the flowchart of Figure 2, a 3D point cloud was obtained using a white light 3D scanning system (Steinbichler—COMET 5®), and then an appropriate software was applied to replicate the 3D ...
3.6. Magnetic nanoscale MOF as potential anticancer drug delivery system, and imaging and MRI contrast agent The combination of both imaging and therapeutic agents in the same MOF greatly facilitates the efficacy studies of theranostic nanoparticles. Having this in mind, Chowdhuri et al. developed a...
Video kinematic parameters in patients underwent CABG. We then moved to experimental medi- cine by adapting our system to an operating room with the purpose of recording the beating hearts of ten patients that underwent CABG (Fig. 6). In average, the coronary occlusion for ten patients ...
Real-time PCR to quantify the mRNA of osteoblastic and adipocityc markers was performed using a CFX96 Real-time PCR Detection System (Bio-Rad; Hercules, CA, USA). The HPRT gene was used to standarize mRNA expression in each sample. Gene expression was quantified using the 2-ΔΔ...
Interestingly, we observed strong coor- dinated effects at other non IFN-regulated genes implicated in the immune system such as CD3D, FGR, PILRA or NLRC4, and in genes with unrecognized function in SS, such as MDGA1, PM20D1 and SIRPB2 (Pearson's coefficient > 0.80). Genes ...