Pull-down Assays for Active Rho. For pull-down of active Rho, Rhotekin-Rho binding domain beads were used according to the instructions from the manufacturer (Cytoskeleton Inc.). In Vitro Kinase Assay and In Vitro Lipid Kinase Assay. Protein kinase assays were carried out by adding ...
Western blot hybridization was carried out using Bio-Rad's Western blotting systems accord- ing to the manufacturer's instructions. Measurement of amino acids and crude protein. High-performance liquid chromatography (HPLC) was used to determine the content of 17 amino acids. Both 2-month-old...
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Mutation Explanation MutaPLM Providing textual explanations of a single-site substitution mutation on a protein sequence Mutation Engineering MutaPLM Generating a mutated protein to fit the textual instructions on the wild-type protein sequence. Protein UniProtID Request N/A Obtaining a protein sequence ...
AbstractNitrogen (N) assimilation is crucial for the growth and development of C3plants, as it converts inorganic N into organic forms, important for protein synthesis, nucleic acids and other vital biomolecules. However, abiotic stressors such as drought, salinity, extreme temperatures and others ...
Protein-protein Interaction PredictionSHS27k, SHS148k, STRINGPIPR, GNN-PPI, OntoProtein Installation (Optional) Creating conda environment: conda create -n OpenBioMed python=3.9 conda activate OpenBioMed Install required packages: pip install -r requirements.txt ...
www.nature.com/scientificreports OPEN Recombinant expression and subcellular targeting of the particulate methane monooxygenase (pMMO) protein components in plants Tatiana Spatola Rossi 1, A. Frances Tolmie 1, Tim Nichol 2, Charlotte Pain 1, Patrick Harrison 3,...
Upon challenge 7 days later, in this case through footpad injection of Candida albicans purified protein, antigen presenting cells carry processed and displayed Candida derivative Scientific Reports | (2019) 9:965 | https://doi.org/10.1038/s41598-018-37175-5 5 www.nature.com/scientificreports/ ...
www.nature.com/scientificreports OPEN Screening for protein-protein interactions using Förster resonance energy transfer (FRET) received: 23 February 2016 and fluorescence lifetime imagingaccepted:19May2016 microscopy (FLIM)Published:24June2016 Anca Margineanu1, Jia Jia Chan2,*, Douglas J....
Morgado1, Daniella Areas Mendes-da- Cruz3, Wilson Savino3, Myriam Sánchez-Gómez2 & Patricia Cuervo1 Protein malnutrition, the most deleterious cause of malnutrition in developing countries, has been considered a primary risk factor for the development of clinical visceral leishmania...