7T, Siemens Healthcare, Erlangen, Germany) using a 24-channel head coil (Nova Medical, Wilmington, pulse cluster aMs Ade,sUcrSiAbe).dTihneSMchRuseenqkueeentcael.u11seadndfosrhTo1wρ-nbainseFdiMg. R5cI consists of an followed by a adiabatically prepared spin-lock conventional MRI readout...
Furthermore, Echo-MRI of mice at 8 weeks of ND or HFD feeding demonstrated that lean phenotype of IEX-1−/− mice was due to less gain in fat mass without affecting lean mass, providing another evidence for enhanced thermogenesis in these mice (Fig. 5H). These findings suggest that...
Tissue collection and sample processing. For collection of corpus callosi tissue, untreated CD1 mouse (Charles River, Wilmington, MA) brains at postnatal ages 3 (P3), P7, P10, and P15 (1 male, 2 females at each stage) were dissected from the skull and placed dorsal surface down on ...
RNA concentrations were measured using a NanoDrop instrument (NanoDrop, Wilmington, DE, USA). Reverse transcription to cDNA was performed using a High-Capacity cDNA Archive Kit (ABI, Foster City, CA, USA). Primers were designed and purchased from Sangon (Sangon, Shanghai, China; see Supporting...