200mM Na+phosphate buffer (pH 7.3), and 0.44g NaCl. To make 10mL of 200mM Na+phosphate buffer (pH 7.3), mix together 2.3mL of 200mM monobasic sodium phosphate and 7.7mL of 200mM dibasic sodium phosphate. Part B consists of a 3.3μM stock solution of rhodamine-labeledphalloidindissolved...
Detection of immunolabeled proteins was performed using chemiluminescence (NEN Life Science Products). Western blots were incubated overnight with primary antibodies against p-AKT (Ser473, #3787), AKT (#9275), p-ERK1/2 (Thr202/Tyr204, #9101), ERK1/2 (#4695) (all pRb; 1:1000, Cell ...
Precisely, 1 mL of each sample’s extract, and 2.5 mL of DPPH solution (0.1 mM), were mixed in labeled test tubes, and incubated in dark for 30 min at room temperature. The absorbance was measured at 517 nm, and percentage inhibition of DPPH radical was calculated by the formula: ...