the 2nd assay is decreased attendant upon the augmentation of concentration of the 2nd marker it seems that shows the state of offer of the individual or the blood by comparing the relative size of the said two signals, in regard to the phase of recovery from the said disease, it is possi...
Here, we report development of a one-pot assay that reconstructs the entire Mtb Mur pathwayin vitroand has the advantage of eliminating the requirement for nucleotide intermediates in the pathway as substrates. The MurA-MurF enzymes were purified and a one-pot assay was developed through ...
近日,中国农业科学院农业基因组研究所阮珏团队在aBIOTECH上发表了题为"A sensitive one-pot ROA assay for rapid miRNA detection"的研究论文,开发了一种基于滚环扩增的一锅等温检测方法ROA,用于快速和特异性检测miRNA和短RNA片段。 该方法将靶标的识别,线性滚环扩增(linear RCA)和引物生成(primer generation)整合到一...
Here, we report development of a one-pot assay that reconstructs the entire Mtb Mur pathwayin vitroand has the advantage of eliminating the requirement for nucleotide intermediates in the pathway as substrates. The MurA-MurF enzymes were purified and a one-pot assay was developed through ...
Biocatalyst activity was defined as the amount of derivative (g) producing 1 µmol/min (IU) of IMP under the assay conditions. All of the determinations were carried out in triplicate, and the maximum error was less than 5%. 3.5. Biochemical Characterization of Immobilized Biocatalysts The pH...
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The control of viral outbreaks requires nucleic acid diagnostic tests that are sensitive, simple and fast. Here, we report a highly sensitive and specific one-pot assay for the fluorescence-based detection of RNA from pathogens. The assay, which can be performed within 30–50 min of incubatio...
Moreover, as evidenced by hemolysis assay, this material exhibited only a negligible hemolysis effect. These results suggest that the composite developed in this work can be used as a promising adsorbent in blood purification application to mitigate the risk of excess bilirubin....
TPB assay (Figure 2A) showed no significant inhibitory effect of Cur ND on 4T1 cells, while that of Dox ND was not even reach IC50 even at the Dox concentration of 20 μg/mL, which suggested the high resistance of 4T1 cells to Dox. However, after treatment with Cur-Dox ND, the ...
In one pot, the self-assembly of AgNO3 and 2-chloroquinoxaline (2Cl-quinox) in water–ethanol mixture afforded two novel crystalline Ag(I) complexes. The major product is the polymeric complex [Ag(2Cl-quinox)(NO3)]n; (1), while the minor product (2) comp