In particular, the C-terminus of one subunit interacts with the N-terminus of the other subunit, constituting one DNA-binding site; the other two termini form the second binding site of the dimer. One binding site is sufficient to bind cruciform DNA. The concerted mechanism involving termini ...
Differential scanning calorimetry on the human SOD1 zinc derivatives also showed increased thermostability of the protein due to zinc binding. Further, the melting transitions observed for the one-zinc derivative closely resembled those of the two-zinc derivative. Finally, we observed that the ...
An cDNA (AtRPB15.9) that encoded a protein related to the RPB4 subunit in yeast RNA polymerase II was cloned. The predicted molecular mass of 15.9 kDa for the AtRPB15.9 protein was significantly smaller than 25 kDa for yeast RBP4. In SDS-PAGE, AtRPB15.9 migrated as the seventh or eighth...
Though the term protein species had been used earlier in the literature [3, 4], it was not clearly defined and used more in the sense of a protein complex consisting of several subunits [3] or to differentiate between different proteins (e.g. catalase and actin were two different protein ...
Using the inner mitochondrial membrane subproteome as a model proteome, we compared for the first time the ability of three protein separation methods (two-dimensional liquid chromatography using the ProteomeLab™ PF 2D Protein Fractionation System from Beckman Coulter, one-dimensional reversed phase ...
Summary Kinesin superfamily motor protein 17 (KIF17) is a candidate transporter of N-methyl-D-aspartate (NMDA) receptor subunit 2B (NR2B). Disruption of th... Xiling,Yin,and,... - 《Neuron》 被引量: 182发表: 2011年 Autoinhibition of the kinesin-2 motor KIF17 via dual intramolecular mech...
Searching for somatic mutations in McCune-Albright syndrome: a comparative study of the peptidic nucleic acid versus the nested PCR method based on 148 DNA... Activating mutations of the Gsalpha gene (GNAS), which encodes for the alpha-subunit of the stimulatory G protein, have been identified...
Marie, J., Simon, M.-P., Dreyfuss, J.-C., and Kahn, A., 1981, One gene but two messenger RNA’s encode liver L and red cell L’ pyruvate kinase subunites, Nature 292 :70–72.Marie J, Simon MP, Dreyfus JC, Kahn A. One gene, but two messenger RNAs encode liver L and red...
Trichothecenes, such as deoxynivalenol (DON), are known to inhibit the protein synthesis in vitro by binding at the 60S subunit of eukaryotic ribosomes. Therefore, cells and tissues with high protein turnover, such as lymphocytes and the liver (albumin and fibrinogen synthesis), were suggested ...
(vWF) expressed on endothelial cells and/or bound to collagen, at sites of injury [41,42,43]. Deficiency of any of the complex subunits, such as (GP)Ibα and (GP)Ibβ, – with the exception of the (GP)V subunit– results in complete absence of the (GP)Ib-IX-V complex, ...