The One Step PrimeScript RT-PCR Kit (Perfect Real Time) is designed for one step, real-time reverse transcription PCR (RT-PCR) using probe detection. With this kit, all RT-PCR steps can be performed in a single tube. Therefore, it is not necessary to add additional reagents during the ...
本试剂盒经过特别的优化,将 Poly(A)加尾反应和cDNA合成反应同步进行,在1小时内即可完成miRNA1stStrandcDNA合成,且产物 无需进行纯化,可直接使用PCR反应试剂SYBRPremixExTaqII(PerfectRealTime)(TaKaRaCode: DRR081)进行RealTimePCR定量检测,从而准确简便地对miRNA(或mRNA、snoRNA等其它RNA分 子)进行表达量分析。由于本...
The One Step PrimeScript™ RT-PCR Kit (Perfect Real Time) or One Step SYBR® PrimeScript™ RT-PCR Kit II (Perfect Real Time) was used to perform RT-qPCR analysis according to the manufacturer’s instructions under the following conditions: reverse transcription (42 °C for 10 min; 95...
PCR Procedure was as follows. The RNA solution was extracted using a simple filter paper-based method [13]. The RNA solution was stored at − 80 °C until use, and 1 µl of the supernatant was used as the template. Using PrimeScript One-Step RT-PCR Kit Ver.2 (Takata Bio, S...
我们合成一步合作使用primescript??? 自然资源部综合套件[合作]宝工业。 成熟的表达式的"和平"号轨道是由214sybr绿色定量实时PCR放大在ABI7500超线程(HT)文书[应用生物系统公司]。 用于数据分析、循环阈值[CT]U6[参考]和MIR214[示例]是在一九六八年四月确定[显示为算术平均值]。 相对的计算表达式是使用相对量化[RQ...
品牌: Jinpan 暂无详情 产品简介 ECEINECS 201-986-7 MDLMFCD00006866 别名7-羟基-4-甲基香豆素 英文名称Hymecromone CAS90-33-5 分子式C10H8O3 分子量176.17 纯度HPLC≥98% 单位瓶 生物活性4-Methylumbelliferone 是一种透明质酸生物合成抑制剂,具有抗肿瘤和抗转移作用[1]。
1one-TimePad 一次一密(一次性便笺密码)是一种理论上无法破 译的加密系统,被誉为“密码编码学的圣杯” 1918年前后由GibertVernam以及Joseph Mauborgne发明 密钥和明文具有相同的长度 密钥不可重复使用,否则就有被破译的可能 收发双方各有一个可以一页一页撕下的本子 ,其中每页上记录一个密钥。类似日历的使 用过程...
One-step real-time RT-PCR assay was performed with the final volume of 20 μl using the One-step PrimeScript RT-PCR Kit (TaKaRa Biotech, Dalian, China) according to the manufacturer’s instructions. Each reaction was carried out using 2 μl of total RNA from collected samples on the Bio...
2.2.15. RNA Isolation and Quantitative Real-Time PCR (qRT-PCR) The total RNAs from the cells were purified using the RiboEx Total RNA solution (GeneAll Biotechnology, Seoul, Korea) and the cDNA was synthesized from the total RNA (2 µg) using SuPrimeScript RT Premix with random hexamer ...
Then, 500 ng of RNA was used to make cDNAs with the PrimeScript RT Master Mix (Cat#RR036A, Takara Bio), according to the manufacturer’s instructions. The cDNAs were diluted at ×10 using Milli-Q water and subjected to qPCR assay based on SYBR Green dye. The target genes were markers...