Moreover, the concentrations of ARPs were measured with a QX100 system (Bio-Rad) using a One-step ddPCR Supermix for Probes kit (Bio-Rad), specific primers, and suitable probes as per the manufacturer’s instructions. Table 2. ARPs used for evaluation of the assay LOD. ID of ARPStrain...
[Objective]The paper was to establish a one-step reverse transcriptase droplet digital PCR(RT-ddPCR)assay for bovine viral diarrhea virus(BVDV).[Method]Based on one-step real-time quantitative PCR(RT-qPCR)assay,BVDV-specific primers and probes were designed in this study.The reverse ...
(INSTAND) were prepared in heat-inactivated stabilized negative saliva 5× diluted in the extraction buffer. The dilutions were freshly prepared before the analysis. Values were assigned to positive controls using a one-step RT-ddPCR advanced kit for probes (BioRad) with N1 and N2 primer–...