For detection of targeted insertion of transgene in fetuses and newborns, genomic DNA was isolated from tail or ear piece using 50 μl of Allele-In-One Mouse Tail Direct Lysis Buffer (#ABP-PP-MT01500; Allele Biotechnology, San Diego, CA). PCR was performed in a total of 10 μl solutio...
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The cell wall occurs in one piece and is smooth. Young cells have a single parietal plastid; older cells have many plastids and nuclei. Reproduction occurs by autospores and zoospores; aplanospores also have been observed (Moore and Carter, 1926). There have been many reports of this genus...
One of the most interesting applications consists of coupling a cataluminescence (CTL) sensor with HS-SPME. The extraction phase was based on ionic liquids (ILs) distributed evenly in a piece of filter paper (planar geometry). This new method was developed for the quantitative determination of ...
Nonetheless, given its historical importance, each newly recovered DNA sequence adds an important piece to the genomic and cultural puzzle of a war-stricken region currently under difficult access. We have been able to infer that the two Umayyad Era individuals buried on top of a prehistoric site...
1916. This watershed date brings to mind a piece of veterinary and medical history from the Vietnam War – a history that links an outbreak of canine disease in Southeast Asia to human disease in the United States. The story emphasizes the importance of collaboration between physicians and veter...
(0.25% trypsin-EDTA diluted 1:2 in DMEM) for 15–25 min resulted in the detachment of an intact layer of cells in one piece with microglial cells remained attached to the bottom of the well; those detached cells were plated and cultured to achieve confluency, and the above methods with ...
Vitamin D-binding protein: one more piece in the puzzle of acromegalic osteopathy? Endocrine. 2016;52(2):183–6. https://doi.org/10.1007/s12020-016-0890-0 (Epub 2016 Feb 22 PMID: 26899438). Article CAS PubMed Google Scholar Jones G, Prosser DE, Kaufmann M. 25-Hydroxyvitamin D-24-...
t know where Billyard contracted the virus, or whether Glead brought it home from work. They talk lightly about an imaginary prison guard from the outbreak-ridden Mission Institution coughing on Billyard in the liquor store, a theory that serves to complete at least one piece of the...