The small size and bright luminescence of NanoLuc® luciferase bring exquisite sensitivity to many applications. From NanoBRET® technology, a BRET-based method to study protein:protein and protein:small molecule interactions, to NanoLuc® Binary Technology (NanoBiT), NanoLuc is the cornerstone for...
To determine how many ATP and NADPH molecules are required to make one molecule of glucose through the Calvin cycle, we can follow these steps:1. Understanding the Calvin Cycle: - The Calvin cycle, also known as the C3 cycl
is required for ATP-dependent, non-lysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. ...
Energy is used inside the body system when the ATP gets hydrolyzed and the total energy is released. The total energy that is present in an ATP molecule helps in the formation of products after a particular reaction. The usage of energy occurs according to the requirement only so that th...
8c), a protein required for assembly of the RS3 head and therefore necessary for male fertility in mice24. Whether domain II of AK8 and domains I and IV of AK9 can cycle between open and closed conformations is unclear, but the remaining catalytic domains would, in principle, be ...
Previous single-molecule studies have demonstrated that MutS diffuses one-dimensionally along a homoduplex DNA (not containing a mismatch) and that ATP hydrolysis is not required at this initial step of the MMR (Gorman et al., 2007). By modeling the diffusive movement of a quantum-dot tagged...
Binding of just one ATP molecule has been shown to be sufficient to induce conformational change across the dimer =-=(20,21)-=-. Crystal structures of the N-terminal domains of GyrB and yeast topoII have been solved, and these both show two subdomains. Subdomain 1 contains an ATP-...
How many total ATP molecules are formed from one glucose molecule during glycolysis, Kreb's cycle and electron transport?ATP:ATP stands for adenosine triphosphate. It is an energy rich phosphate and links energy requiring and energy producing reactions. I...
We show that TRiC is among the sperm-specific additions to the axoneme. As a chaperone for tubulin folding, TRiC contributes to the assembly of the extensive microtubule cytoskeleton of the ciliary axoneme and is required for ciliogenesis inTetrahymena50and for spermiogenesis inPlanaria51. Anchori...
In terms of ATP cost, this process is far more costly than amino acid synthesis (some require one ATP molecule, some zero) and is comparable to some lipids (such as palmitoyl-CoA, although those require also high number of NADPH molecules) (8). This means that cellular commitment to this...