The study demonstrates that monoallelic expression is much more widespread than previously though, implying that variation of an epigenetic nature might have important consequences for studies of human disease susceptibility.doi:10.1038/nrg2287Skipper...
Characterization ofPtUMPSknock-out strains generated using a TALE nuclease.aStructure of thePtUMPSlocus. The exons are represented as black boxes, with the putative catalytic sites in red and the TALEN-targeted site in blue. The half-sites recognized by each TALEN are boxed.bSequence chromatogram...
One-allele system in the Korean for MboI-RFLP in exon 1 of the human tyrosinase (TYR) genedoi:10.1016/S0923-1811(00)00075-XAlbinismTyrosinaseMboI-RFLPOculocutaneous albinism is an autosomal recessive genetic disorder. Several types of oculocutaneous albinism are caused by mutation in related ...
The development of single-cell RNA-seq methods has shown significant progress owing to the fact the each single cell harbors thousands of copies of each mRNA transcript, while having only two copies of each chromosomal DNA molecule. Consequently, the field has seen a proliferation of methods for...
1.A linear strand of DNA and associated proteins in the nucleus of eukaryotic cells that carries the genes and functions in the transmission of hereditary information. 2.A circular strand of DNA in bacteria and archaea that contains the hereditary information necessary for cell life. ...
the target detection module, in addition to the CheckV quality score, percentage genome completeness, and contig length, if Entourage is used for read assembly, this module also reports information on the number of supporting reads, back-mapping coverage, and average sequencing depth for each ...
Four exons of Per2 were targeted (Fig.聽2A).Figure 2Efficient targeting of Bmal1 and Per2 genes in U2OS cells by the all-in-one CRISPR-Cas9 vector. (A) Four exons were targeted in each gene based on their score in a predictive software tool. The PAM sequences are indicated in red....
Hereditary progressive dystonia with marked diurnal fluctuation (HPD; dopa-responsive dystonia, DRD) have been recently found to be caused by a genetic defect in the GTP cyclohydrolase I (GCH1) gene. In this study, we quantified the mRNA level of GCH1 in phytohemagglutinin (PHA)-stimulated mono...
The combination of knocking one allele out with CRISPR-mediated NHEJ and targeting the other with a conditionally inactivating cassette allows rapid generation of conditional alleles. Loss-of-function studies are key for investigating gene function, and
Site-specific insertion of a 2.7 kb CreERT2 cassette into the mouse Nfatc1 locus allowed labeling and tracing of hair follicle stem cells. In addition, we combined the Cre- Loxp system with a gene-trap strategy to insert a GFP reporter in the reverse orientation into the rat Lgr5 locus...