Signal amplificationThe high-sensitive and rapid detection of critical biomarkers, e.g., disease-related nucleic acids and proteins, is always desired. Compared with the routine homogenous detection strategies, the on-bead flow cytometry (FCM)-based assays have drawn a lot of interests owing to ...
ChIP-grade antibodies ChIP-seq crucially depends on the antibody used for the immunoprecipitation: it must recognize its epitope under conditions where fixatives have chemically modified primary amines. Therefore, antibodies that have previously worked in ChIP-seq are most likely to generate favorable ou...
NLM and flow cytometry analysis of the MBA assays for biotinylated bovine serum albumin and double-stranded DNA. (a) Optical micrographs of the separation process. (i) Single beads and dimers travel on the array of S-micromagnets at low driving frequency, (ii,iii) when the driving frequency ...
Validation of Flow Cytometry and Magnetic Bead-Based Methods to Enrich CNS Single Cell Suspensions for Quiescent Microglia Microglia are resident mononuclear phagocytes within the CNS parenchyma that intimately interact with neurons and astrocytes to remodel synapses and extrac... T.,A.,Volden,... ...
Compar- ison of the sorting efficiency and influence on cell function between the sterile flow cytometry and immunomagnetic bead purification methods. Prep Biochem Biotechnol 43, 197, 2013.Li Q, Zhang X, Peng Y, Chai H, Xu Y, Wei J, et al. Comparison of the sorting efficiency and ...
Bead-based assayFlow cytometryReal-time monitoringMicrobeaconURACIL-DNA GLYCOSYLASEMOLECULAR BEACONSESCHERICHIA-COLICARCINOMA-CELLSLABEL-FREEAs the support of all living kingdoms' genetic information, the integrity of the DNA biomolecule must be preserved. To that goal, cells have evolved specific DNA ...
It contains reagents for measuring particular sets of cytokines using flow cytometry to determine if plasticity toward a Th1, Th2, or Th17 response may be occurring during T-cell differentiation. The BD FACSVerseTM system includes the cytometer and BD FACSuiteTM software for acquisition and ...
Signal-on/signal-off bead-based assays for the multiplexed monitoring of base excision repair activities by flow cytometrydoi:10.1007/s00216-021-03849-9Analytical and Bioanalytical Chemistry - As the support of all living kingdoms' genetic information, the integrity of the DNA biomolecule must be ...
Comparison of FISH analysis and BCR-ABL1 Flow-cytometry Bead Assay on CML samples (N = 105 serial samples in 37 patients).Anna, Grazia Recchia
Flow cytometryImmunophenotypingMagnetic sortingMultiple myelomaWhole bone marrowCD138 magnetic sorting impacts the phenotypic characteristics of myeloma plasma cells. In our evaluation of bone marrow aspirates from 16 patients, we observed complete loss or under expression of cell surface antigens by CD138...