OBRC——生物医药研究工具 | OBRC是一种生物药物研究领域常用的技术,全称为Oligonucleotide-Based Ribosome Profiling(寡核苷酸基于核糖体剖析)。它是一种高通量的转录组学方法,用于研究细胞中的转录和翻译过程。OBRC的原理是通过将细胞中的RNA与寡核苷酸标记结合,然后利用核糖体剖析技术来测量RNA的翻译效率和转录水平。
Achenbach TV,Brunner B,Heermeier K.Oligonucleotide-based knockdown technologies:antisense versus RNA interference. ChemBioChem . 2003Achenbach TV,Brunner B,Heermeier K.Oligonucleotide-based knockdown technologies:antisense versus RNA interference.ChemBioChem. 2003Achenbach T, Brunner B, Heermeier K. ...
Oligonucleotide-Based Drugs and Therapeutics:Preclinical and Clinical Considerations for Development-- 1 反义基础的药物和疗法:发展的临床前和临床考虑 EISBN:9781119070290 ISBN:9781118537336 出版年:2018 页数:578 出版社:Wiley 语种:英语 参考价格:USD 231.95 ...
Purpose:The most frequent genetic cause of Leber congenital amaurosis (LCA) is an intronic change in CEP290, that results in the insertion of a cryptic exon (exon X) into the CEP290 mRNA. Recently, we have shown promising results of antisense oligonucleotide (AON)-based therapy for this ...
Oligonucleotide-based knockdown technologies: antisense versus RNA interference. Chembiochem. 2003; 4: 928-35.Achenbach TV, Brunner B, Heermeier K (2003) Oligonucleotide- based knockdown technologies: antisense versus RNA interfer- ence. Chem Bio Chem 4(10):928-935. doi:10.1002/cbic.2003 00708...
Alternatively, anti-dsDNA antibodies can be demonstrated by immunofluorescence, using the haemoflagellate Crithidia luciliae (CLIFT).%We have developed a new oligonucleotide-based anti-dsDNA ELISA in which dsDNA, constituted of a 5鈥瞤hotobiotinylated nucleic acid probe and its highly specific ...
5Ј33Ј exonucleases in phosphorothioate-based oligonucleotide-directed mutagenesis. Nucleic Ac- ids Res. 16:791-802.Sayers et al. (1988). " Y-T Exonucleases in phosphorothioate-based oligonucleotide-directed mutagenesis. " Nucleic Acids Res. 16:791-802....
When the RNA A-form helical structure is fully base-paired, the RNA grooves are sterically inaccessible and difficult to target using small molecules or peptide-based therapeutics. Nevertheless, structural studies suggest that perturbations in the A-form RNA helix can lead to the formation of ...
Label-free luminescent oligonucleotide-based probes. Chem. Soc. Rev. 42, 3427-3440. http://dx.doi.org/10.1039/c2cs35472a.Ma DL, He HZ, Leung KH, Zhong HJ, Chan DSH, Leung CH. Label-free luminescent oligonucleotide-based probes. Chem Soc Rev, 2013, 42: 3427-3440...
Establishment of oligonucleotide-based DNA microarrays of the Gluconobacter oxydans 621H genome: first results on the influence of pH and oxygen on the transcriptomeEscherichia coliMethotrexateFolic AcidTetrahydrofolatesTetrahydrofolate DehydrogenaseOxygenases...