S., Ding, Q., Collins, R., Ragavendran, A., Brand, H., et al. (2014). Low incidence of off-target mutations in individual CRISPR- Cas9 and TALEN targeted human stem cell clones detected by whole- genome sequencing. Cell Stem Cell 15, 27-30. doi: 10.1016/j.stem.2014. 04.020...
In a previous study, we used CRISPR/Cas9 to edit the grapevine genome and obtained 22 edited lines with no off-target mutations detected by target sequencing12. To characterize the potential genome-wide off-target rate in greater depth, in this study, we performed a large-scale WGS analysis ...
CAS9 nucleases, a new tool for genome editing, show significant offtarget activity. Clustered, regularly interspaced, short palindromic repeat (CRISPR) RNA-guided nucleases (RGNs) have rapidly emerged as a facile and efficient platform for genome editing
利用CRISPR/CAS9技术在生殖细胞中治愈小鼠遗传疾病(英文) cure the resulting cataracts disease in the offspring with 100%efficiency.Importantly,no off-target mutations were identified through whole-genome sequencing.doi... 李大力,Dali,Li,... - 《Science Bulletin》 被引量: 0发表: 2015年 加载更多来源...
Five monkeys were ultimately gestated, with four surviving delivery. All the offspring monkeys displayed successful CRISPR modifications. Several techniques revealed that there were in fact no detectable off-target effects, but some de novo mutations were ultimately identified. Close study of the partic...
In addition, paired Cas9 nickases, composed of D10A Cas9 and guide RNA, which generate two single-strand breaks (SSBs) or nicks on different DNA strands, were highly specific in human cells, avoiding off-target mutations without sacrificing genome-editing efficiency. Interestingly, paired nickase...
Recently developed DNA base editing methods enable the direct generation of desired point mutations in genomic DNA without generating any double-strand breaks1–3, but the issue of off-target edits has limited the application of these methods. Although s
An unbiased approach for the genome-wide detection of off-target cleavage by CRISPR-Cas9 RNA–guided nucleases reveals wide variability in the off-target activity of different guide RNAs. CRISPR RNA-guided nucleases (RGNs) are widely used genome-editing
CRISPR/Cas9 nucleases are widely used for genome editing, but they frequently induce unwanted off-target mutations [1]. The specificity of the CRISPR system is largely determined by how specific the single-guide RNA (sgRNA) targeting sequence is when compared to the rest of the genome for the...
CRISPR/Cas-derived RNA-guided engineered nucleases (RGENs) enable genome editing in cultured cells, animals and plants, but are limited by off-target mutations. Here, we present a novel algorithm termed Cas-OFFinder that searches for potential off-target sites in a given genome or user-defined...