iPSC创建的核心是转入四个转录因子:Oct4,Sox2,Klf4和cMyc(OSKM),它们也被称为山中因子。 图片来源:pixabay.com 山中因子这个组合中,cMyc可以去掉,但是会影响重编程的效率[1],Sox2和Kfl4也可以被家族中的其他因子取代,但唯独Oct4是难以被取代,也无法去掉的。过去的研究中,尝试不让Oct4过表达来创建iPSC的,无一...
Yamanaka 实验室的后续工作表明,在重编程混合物中的四种 TF——Oct4、Sox2、Klf4 和 cMyc (OSKM)——中,只有 cMyc 可以省略,同时仍然允许产生诱导多能干细胞(iPSC) (Nakagawa 等人, 2008)。此外,尽管 Sox2、Kfl4 和 cMyc 可以被其 TF 家族的其他成员取代,但 Oct4 不能被 Oct1 或 Oct6 取代。已经进行...
这个事件的科学背景是诺贝尔奖Yamanaka及其提出的转录因子 (Sox2, Oct3/4, Klf4 and c-Myc; or OSKM) ,也称“中山因子”,详见:网页链接 2022-01-20 19:14 作者:安东尼奥-雷加拉多 去年10月,一大群科学家来到尤里-米尔纳位于帕洛阿尔托上方洛斯阿尔托斯山的超级豪宅。他们接受了新冠肺炎的测试,并戴着口罩,聚集在...
The four OSKM factors OCT4, SOX2, KLF4 and c-MYC are key transcription factors modulating pluripotency, self-renewal and tumorigenesis in stem cells. However, although their transcriptional targets have been extensively studied, little is known about how these factors are regulated at the post...
在2006年,日本科学家山中伸弥(Shinya Yamanaka)发表诱导性多能干细胞(iPS, induced pluripotent stem cells)构建技术,利用逆转录病毒,把OSKM(OCT4, SOX2,KLF4 and C-myc)四因子在体细胞中进行超表达,成功把小鼠胚胎成纤维细胞诱导形成多能干细胞,开辟了一个全新的干细胞研究领域。随着研究的深入,人皮肤成纤维细胞...
Figure 1. Sox2, Klf4, and cMyc Can Reprogram to Pluripotency in the Absence of Exogenous POU Factor (A and C) Scheme of KSM and SKM polycistronic vectors derived from OKSM (A) and OSKM (C) reprogramming cassettes. (B and D) Generation of iPSCs with tetO-KSM (B) and SKM (D) vec...
Oct34, Klf4, c-Myc and Sox2 produce induced pluri 优质文献 相似文献 参考文献 引证文献Induction of Pluripotent Stem Cells from Mouse Embryonic and Adult Fibroblast Cultures by Defined Factors pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2,...
2、g)。在2006年,日本科学家山中伸弥(Shinya Yamanaka)发表诱导性多能干细胞(iPS, induced pluripotent stem cells)构建技术,利用逆转录病毒,把OSKM(OCT4, SOX2,KLF4 and C-myc)四因子在体细胞中进行超表达,成功把小鼠胚胎成纤维细胞诱导形成多能干细胞,开辟了一个全新的干细胞研究领域。随着研究的深入,人皮肤成...
Expression of the four transcription factors Oct4, Sox2, Klf4, and c-Myc (OSKM) is sufficient to reprogram somatic cells into induced pluripotent stem (iPS... F Gerrit,DC Klein,G Wu,... - 《Plos One》 ...
Mouse embryonic fibroblasts (MEFs) have been successfully reprogrammed into induced pluripotent stem cells (iPSCs) through the ectopic expression of the transcription factors Oct4, Sox2, Klf4, and cMyc (OSKM)1. Exploring this potential further, several groups achieved similar reprogramming from various...