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while a C-language code was used to post-process the data. The resulting data matrix for each camera pixel was then fitted with a two-exponential functionI (t) = A1 exp (-t/τ1) + A2 exp (-t/τ2)in Tri2 software46to determine lifetime values. More details of data proce...
yielded a pixel size of 1.36 Å or 1.32 Å on images (Extended Data Table1). The defocus range was set to between −1.2 and −2.2 μm. All movie stacks were collected by SerialEM under a dose rate of 9.1–9.4 e−/pixel/s with a total exposure time of 11.4...
The three-axis stabilized camera can capture 4K videos and 13 megapixel photos and is equipped with advanced digital zoom. The aircraft sensors support intelligent obstacle avoidance, visual tracking, gesture recognition, and other advanced functionality. 1. 3 axis Stabilized Camera 7. Propellers 2....
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and from these frames emission decay is reconstructed and lifetime is determined for each pixel of the CCD matrix. By applying a probe calibration function (determined in a separate experiment), lifetime images of the sample can be converted into an O2concentration map. For the laser-scanning ...